Cloned (Comment) | Organism |
---|---|
gene pyk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | Mycobacterium tuberculosis | - |
ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | Mycobacterium tuberculosis H37Rv | - |
ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | Mycobacterium tuberculosis ATCC 25618 | - |
ATP + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKE5 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WKE5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WKE5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.31 | - |
purified recombinant His-tagged enzyme, pH 7.6, 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Mycobacterium tuberculosis | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | - |
Mycobacterium tuberculosis H37Rv | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | - |
Mycobacterium tuberculosis ATCC 25618 | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
More | secondary structure analysis by circular dichroism spectroscopy revealing a content of 17% alpha-helix, 34% beta-sheet, and 49% turns in the enzyme | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PYK | - |
Mycobacterium tuberculosis |
Rv1617 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Mycobacterium tuberculosis |