Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
at pH 7.0 and 70°C | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerol | - |
Thermococcus kodakarensis | ADP + sn-glycerol 3-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
when the enzyme fused with either polyphosphate kinase of Thermus thermophilus HB27 or an Escherichia coli membrane-intrinsic protein YedZ is incubated at 70°C for 2 h, more than 80% of enzyme activity is retained in the heated Escherichia coli cells | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermococcus kodakarensis |