Literature summary for 2.7.1.236 extracted from

Han, Q.; Zhou, C.; Wu, S.; Liu, Y.; Triplett, L.; Miao, J.; Tokuhisa, J.; Deblais, L.; Robinson, H.; Leach, J.E.; Li, J.; Zhao, B.
Crystal structure of Xanthomonas AvrRxo1-ORF1, a type III effector with a polynucleotide kinase domain, and its interactor AvrRxo1-ORF2 (2015), Structure, 23, 1900-1909 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C41 cells and Xanthomonas oryzae strain X11-5A	Xanthomonas oryzae pv. oryzicola

Crystallization (Commentary)

Crystallization (Comment)	Organism
AvrRxo1-ORF1:AvrRxo1-ORF2 tetramer complex, hanging drop vapor diffusion method, using either 0.8 M NaH2PO4, 0.8 M KH2PO4, 15% (v/v) glycerol, 8 mM dithiothreitol, and 0.1 M HEPES pH 7.5 (native or selenomethione-labeled enzyme) or 1.6 M (NH4)2SO4, 18% (v/v) glycerol, 8 mM dithiothreitol, 100 mM Na-citrate pH 5.0 (sulfate complex)	Xanthomonas oryzae pv. oryzicola

Protein Variants

Protein Variants	Comment	Organism
T167N	ATP-binding site mutant	Xanthomonas oryzae pv. oryzicola

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + NAD+	Xanthomonas oryzae pv. oryzicola	-	ADP + 3'-NADP+	-	?
ATP + NAD+	Xanthomonas oryzae pv. oryzicola BLS256	-	ADP + 3'-NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas oryzae pv. oryzicola	Q6TKR8	-	-
Xanthomonas oryzae pv. oryzicola BLS256	Q6TKR8	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose 4B affinity resin Q-Sepharose ion-exchange column chromatography	Xanthomonas oryzae pv. oryzicola

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + NAD+	-	Xanthomonas oryzae pv. oryzicola	ADP + 3'-NADP+	-	?
ATP + NAD+	-	Xanthomonas oryzae pv. oryzicola BLS256	ADP + 3'-NADP+	-	?
additional information	the enzyme does not possess uridine diphosphate-N-acetylglucosamine kinase activity	Xanthomonas oryzae pv. oryzicola	?	-	-
additional information	the enzyme does not possess uridine diphosphate-N-acetylglucosamine kinase activity	Xanthomonas oryzae pv. oryzicola BLS256	?	-	-

Synonyms

Synonyms	Comment	Organism
AvrRxo1	-	Xanthomonas oryzae pv. oryzicola

General Information

General Information	Comment	Organism
physiological function	the enzyme has a significant virulence function that can increase bacterial growth on the host plant. The enzyme functions to enhance bacterial multiplication in plants, and full virulence function is dependent on its putative kinase activity	Xanthomonas oryzae pv. oryzicola

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Release 2023.1 (January 2023)