BRENDA - Enzyme Database
show all sequences of 2.7.1.226

An archaeal ADP-dependent serine kinase involved in cysteine biosynthesis and serine metabolism

Makino, Y.; Sato, T.; Kawamura, H.; Hachisuka, S.; Takeno, R.; Imanaka, T.; Atomi, H.; Nat. Commun. 7, 13446 (2016)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4
-
ADP
pH 7.5, 85°C
Thermococcus kodakarensis
5.1
-
L-serine
pH 7.5, 85°C
Thermococcus kodakarensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus kodakarensis
Q5JD03
-
-
Thermococcus kodakarensis ATCC BAA-918
Q5JD03
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + L-serine
-
751658
Thermococcus kodakarensis
AMP + O-phospho-L-serine
-
-
-
?
ADP + L-serine
-
751658
Thermococcus kodakarensis ATCC BAA-918
AMP + O-phospho-L-serine
-
-
-
?
additional information
activity is specific towards free serine, and not observed with threonine, homoserine and serine residues within a peptide
751658
Thermococcus kodakarensis
?
-
-
-
-
additional information
activity is specific towards free serine, and not observed with threonine, homoserine and serine residues within a peptide
751658
Thermococcus kodakarensis ATCC BAA-918
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
204
-
ADP
pH 7.5, 85°C
Thermococcus kodakarensis
218
-
L-serine
pH 7.5, 85°C
Thermococcus kodakarensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4
-
ADP
pH 7.5, 85°C
Thermococcus kodakarensis
5.1
-
L-serine
pH 7.5, 85°C
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + L-serine
-
751658
Thermococcus kodakarensis
AMP + O-phospho-L-serine
-
-
-
?
ADP + L-serine
-
751658
Thermococcus kodakarensis ATCC BAA-918
AMP + O-phospho-L-serine
-
-
-
?
additional information
activity is specific towards free serine, and not observed with threonine, homoserine and serine residues within a peptide
751658
Thermococcus kodakarensis
?
-
-
-
-
additional information
activity is specific towards free serine, and not observed with threonine, homoserine and serine residues within a peptide
751658
Thermococcus kodakarensis ATCC BAA-918
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
204
-
ADP
pH 7.5, 85°C
Thermococcus kodakarensis
218
-
L-serine
pH 7.5, 85°C
Thermococcus kodakarensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
42.4
-
L-serine
pH 7.5, 85°C
Thermococcus kodakarensis
86.4
-
ADP
pH 7.5, 85°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
42.4
-
L-serine
pH 7.5, 85°C
Thermococcus kodakarensis
86.4
-
ADP
pH 7.5, 85°C
Thermococcus kodakarensis
Other publictions for EC 2.7.1.226
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749458
Nagata
Structural study on the react ...
Thermococcus kodakarensis, Thermococcus kodakarensis ATCC BAA-918
ACS Chem. Biol.
12
1514-1523
2017
-
-
1
1
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
751658
Makino
An archaeal ADP-dependent ser ...
Thermococcus kodakarensis, Thermococcus kodakarensis ATCC BAA-918
Nat. Commun.
7
13446
2016
-
-
-
-
-
-
-
2
-
-
-
-
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2