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Literature summary for 2.7.1.186 extracted from

  • Motoyama, K.; Sobue, F.; Kawaide, H.; Yoshimura, T.; Hemmi, H.
    Conversion of mevalonate 3-kinase into 5-phosphomevalonate 3-kinase by single amino acid mutations (2019), Appl. Environ. Microbiol., 85, e00256-19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.186

Cloned(Commentary)

Cloned (Comment) Organism
gene Ta0762, the pBAD-TacM plasmid series contains the genes of M3K, M3P5K, BMD, and IPK for the expression of part of modified MVA pathway II. Although the M3K, M3P5K, and IPK genes are derived from Thermoplasma acidophilum, various BMD genes have been utilized for plasmid construction. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum
-
 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum JCM 9062
-
 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum AMRC-C165
-
 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum ATCC 25905
-
 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum NBRC 15155
-
 ADP + 3,5-bisphosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HK44
-
-
Thermoplasma acidophilum AMRC-C165 Q9HK44
-
-
Thermoplasma acidophilum ATCC 25905 Q9HK44
-
-
Thermoplasma acidophilum JCM 9062 Q9HK44
-
-
Thermoplasma acidophilum NBRC 15155 Q9HK44
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum JCM 9062 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum AMRC-C165 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum ATCC 25905 ADP + 3,5-bisphosphomevalonate
-
 ?
ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum NBRC 15155 ADP + 3,5-bisphosphomevalonate
-
 ?

Synonyms

Synonyms Comment Organism
M3P5K
-
 Thermoplasma acidophilum
MVA-3-P 5-kinase
-
 Thermoplasma acidophilum
Ta0762
-
 Thermoplasma acidophilum

General Information

General Information Comment Organism
evolution mevalonate 3-phosphate 5-kinase is an enzyme involved in the modified mevalonate pathway specific to limited species of thermophilic archaea Thermoplasma acidophilum
metabolism mevalonate 3-kinase plays a key role in a recently discovered modified mevalonate pathway specific to thermophilic archaea of the order Thermoplasmatales, pathway overview. In the pathway called modified MVA pathway II, mevalonate (MVA) is phosphorylated at the 3-hydroxyl group to yield 3-phosphomevalonate (MVA-3-P) by the action of mevalonate 3-kinase (M3K, EC 2.7.1.185) rather than at the 5-hydroxyl group as in the reaction of MVK (EC 2.7.4.2). M3K is also homologous to diphosphomevalonate decarboxylase (DMD). After the formation of MVA-3-P, another kinase, MVA-3-P 5-kinase (M3P5K), catalyzes its 5-phosphorylation, and a subsequent decarboxylation is catalyzed by another DMD homologue, 3,5-bisphosphomevalonate decarboxylase (BMD), to give isopentenyl phosphate (IP). IP is then phosphorylated by isopentenyl phosphate kinase (IPK) to yield isopentenyl diphosphate (IPP) Thermoplasma acidophilum
physiological function mevalonate 3-phosphate 5-kinase catalyzes the ATP-dependent 5-phosphorylation of 3-phosphomevalonate Thermoplasma acidophilum