BRENDA - Enzyme Database
show all sequences of 2.7.1.175

Biochemical characterization of the maltokinase from Mycobacterium bovis BCG

Mendes, V.; Maranha, A.; Lamosa, P.; da Costa, M.S.; Empadinhas, N.; BMC Biochem. 11, 21 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
Mak may be a potential drug target in Mycobacterium tuberculosis
Mycobacterium tuberculosis variant bovis
Cloned(Commentary)
Commentary
Organism
gene mak, DNA and amino acid sequence determination and analysis, high level overexpression of His-tagged Mak in Escherichia coli
Mycobacterium tuberculosis variant bovis
General Stability
General Stability
Organism
addition of 50 mM NaCl markedly enhances Mak stability
Mycobacterium tuberculosis variant bovis
Inhibitors
Inhibitors
Commentary
Organism
Structure
glycerol
strongly inhibits Mak activity, even at very low concentrations of 1%
Mycobacterium tuberculosis variant bovis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
recombinant Mak exhibits Michaelis-Menten kinetics at 37°C with ATP, GTP and UTP up to 5 mM
Mycobacterium tuberculosis variant bovis
0.74
-
ATP
pH 8.0, 37°C, recombinant His-tagged Mak
Mycobacterium tuberculosis variant bovis
2.52
-
maltose
pH 8.0, 37°C, recombinant His-tagged Mak
Mycobacterium tuberculosis variant bovis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
activates, 41% activity compared to Mg2+
Mycobacterium tuberculosis variant bovis
Mg2+
best activating cation
Mycobacterium tuberculosis variant bovis
Mn2+
activates, 14% activity compared to Mg2+
Mycobacterium tuberculosis variant bovis
additional information
divalent cations are required for activity and Mg2+ is the best activator
Mycobacterium tuberculosis variant bovis
NaCl
addition of 50 mM NaCl markedly enhances Mak stability
Mycobacterium tuberculosis variant bovis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50700
-
1 * 50700, recombinant His-tagged Mak, SDS-PAGE
Mycobacterium tuberculosis variant bovis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + maltose
Mycobacterium tuberculosis variant bovis
-
ADP + maltose 1-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis variant bovis
-
gene mak
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Mycobacterium tuberculosis variant bovis
Storage Stability
Storage Stability
Organism
4°C, purified recombinant His-tagged enzyme, 7 days, about 40% of activity remaining, at pH 7.5, most of the activity is lost after freeze/thawing at -20°C. 10 mM maltose only slightly improve the stability at 4°C to about 50% of maximal activity, but addition of 50 mM NaCl dramatically improve the stability of the enzyme with over 90% activity remaining after 1 week at 4°C
Mycobacterium tuberculosis variant bovis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
ADP + maltose 1-phosphate
-
-
-
?
ATP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
ADP + maltose 1-phosphate
NMR spectroscopical product analysis
-
-
?
GTP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
GDP + maltose 1-phosphate
NMR spectroscopical product analysis
-
-
?
UTP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
UDP + maltose 1-phosphate
NMR spectroscopical product analysis
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 50700, recombinant His-tagged Mak, SDS-PAGE
Mycobacterium tuberculosis variant bovis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Mycobacterium tuberculosis variant bovis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
65
activity range
Mycobacterium tuberculosis variant bovis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
at 37°C
Mycobacterium tuberculosis variant bovis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
activity range
Mycobacterium tuberculosis variant bovis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
dependent on
Mycobacterium tuberculosis variant bovis
GTP
can substitute for ATP
Mycobacterium tuberculosis variant bovis
UTP
can substitute for ATP
Mycobacterium tuberculosis variant bovis
Application (protein specific)
Application
Commentary
Organism
drug development
Mak may be a potential drug target in Mycobacterium tuberculosis
Mycobacterium tuberculosis variant bovis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene mak, DNA and amino acid sequence determination and analysis, high level overexpression of His-tagged Mak in Escherichia coli
Mycobacterium tuberculosis variant bovis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
dependent on
Mycobacterium tuberculosis variant bovis
GTP
can substitute for ATP
Mycobacterium tuberculosis variant bovis
UTP
can substitute for ATP
Mycobacterium tuberculosis variant bovis
General Stability (protein specific)
General Stability
Organism
addition of 50 mM NaCl markedly enhances Mak stability
Mycobacterium tuberculosis variant bovis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycerol
strongly inhibits Mak activity, even at very low concentrations of 1%
Mycobacterium tuberculosis variant bovis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
recombinant Mak exhibits Michaelis-Menten kinetics at 37°C with ATP, GTP and UTP up to 5 mM
Mycobacterium tuberculosis variant bovis
0.74
-
ATP
pH 8.0, 37°C, recombinant His-tagged Mak
Mycobacterium tuberculosis variant bovis
2.52
-
maltose
pH 8.0, 37°C, recombinant His-tagged Mak
Mycobacterium tuberculosis variant bovis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
activates, 41% activity compared to Mg2+
Mycobacterium tuberculosis variant bovis
Mg2+
best activating cation
Mycobacterium tuberculosis variant bovis
Mn2+
activates, 14% activity compared to Mg2+
Mycobacterium tuberculosis variant bovis
additional information
divalent cations are required for activity and Mg2+ is the best activator
Mycobacterium tuberculosis variant bovis
NaCl
addition of 50 mM NaCl markedly enhances Mak stability
Mycobacterium tuberculosis variant bovis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50700
-
1 * 50700, recombinant His-tagged Mak, SDS-PAGE
Mycobacterium tuberculosis variant bovis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + maltose
Mycobacterium tuberculosis variant bovis
-
ADP + maltose 1-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Mycobacterium tuberculosis variant bovis
Storage Stability (protein specific)
Storage Stability
Organism
4°C, purified recombinant His-tagged enzyme, 7 days, about 40% of activity remaining, at pH 7.5, most of the activity is lost after freeze/thawing at -20°C. 10 mM maltose only slightly improve the stability at 4°C to about 50% of maximal activity, but addition of 50 mM NaCl dramatically improve the stability of the enzyme with over 90% activity remaining after 1 week at 4°C
Mycobacterium tuberculosis variant bovis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
ADP + maltose 1-phosphate
-
-
-
?
ATP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
ADP + maltose 1-phosphate
NMR spectroscopical product analysis
-
-
?
GTP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
GDP + maltose 1-phosphate
NMR spectroscopical product analysis
-
-
?
UTP + maltose
-
717432
Mycobacterium tuberculosis variant bovis
UDP + maltose 1-phosphate
NMR spectroscopical product analysis
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 50700, recombinant His-tagged Mak, SDS-PAGE
Mycobacterium tuberculosis variant bovis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Mycobacterium tuberculosis variant bovis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
65
activity range
Mycobacterium tuberculosis variant bovis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
at 37°C
Mycobacterium tuberculosis variant bovis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
activity range
Mycobacterium tuberculosis variant bovis
General Information
General Information
Commentary
Organism
evolution
genetic environment of the mak gene in different organisms. Organization of the region containing the mak gene, overview
Mycobacterium tuberculosis variant bovis
metabolism
involvement of maltose-1-phosphate in the regulation of sugar metabolism in Escherichia coli
Mycobacterium tuberculosis variant bovis
General Information (protein specific)
General Information
Commentary
Organism
evolution
genetic environment of the mak gene in different organisms. Organization of the region containing the mak gene, overview
Mycobacterium tuberculosis variant bovis
metabolism
involvement of maltose-1-phosphate in the regulation of sugar metabolism in Escherichia coli
Mycobacterium tuberculosis variant bovis
Other publictions for EC 2.7.1.175
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739666
Fraga
Structure of mycobacterial mal ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618, Mycolicibacterium vanbaalenii, Mycolicibacterium vanbaalenii DSM 7251
Sci. Rep.
5
8026
2015
-
-
2
1
15
-
-
-
-
2
-
4
-
6
-
-
2
1
-
-
-
-
4
1
3
-
-
-
2
-
-
2
-
-
-
-
-
2
2
1
15
-
-
-
-
-
-
2
-
4
-
-
-
2
-
-
-
-
4
1
3
-
-
-
2
-
-
-
-
5
5
-
-
-
737320
Roy
Synthesis of alpha-glucan in m ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
ACS Chem. Biol.
8
2245-2255
2013
1
-
1
-
3
-
-
1
-
1
3
2
-
7
-
-
1
-
-
-
-
-
2
2
-
-
-
-
1
-
-
1
-
-
-
1
-
1
1
-
3
-
-
-
-
1
-
1
3
2
-
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
3
3
-
-
-
717432
Mendes
Biochemical characterization o ...
Mycobacterium tuberculosis variant bovis
BMC Biochem.
11
21
2010
-
1
1
-
-
1
1
3
-
5
1
1
-
5
-
-
1
-
-
-
-
1
4
1
1
1
-
-
1
1
-
3
-
-
-
-
1
1
3
-
-
1
-
1
-
3
-
5
1
1
-
-
-
1
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
2
2
-
-
-
717816
Elbein
Last step in the conversion of ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 14468
J. Biol. Chem.
285
9803-9812
2010
-
1
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
717739
Jarling
Isolation of mak1 from Actinop ...
Actinoplanes missouriensis, Streptomyces coelicolor, Streptomyces coelicolor A3(2)
J. Basic Microbiol.
44
360-373
2004
-
-
2
-
-
-
-
-
-
1
4
-
-
9
-
-
2
-
-
-
1
-
3
2
1
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
4
-
-
-
-
2
-
-
1
-
3
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
717163
Niehues
Isolation and characterization ...
Actinoplanes missouriensis
Arch. Microbiol.
180
233-239
2003
-
-
-
-
-
-
-
3
-
1
1
-
-
2
-
-
1
-
-
-
-
-
1
1
1
1
-
-
2
-
-
2
-
1
-
-
-
-
2
-
-
-
-
-
-
3
-
1
1
-
-
-
-
1
-
-
-
-
1
1
1
1
-
-
2
-
-
1
-
-
-
-
-
-
717600
Drepper
Maltokinase (ATP:maltose 1-pho ...
Actinoplanes sp.
FEBS Lett.
388
177-179
1996
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-