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Literature summary for 2.7.1.161 extracted from

  • Mashhadi, Z.; Zhang, H.; Xu, H.; White, R.H.
    Identification and characterization of an archaeon-specific riboflavin kinase (2008), J. Bacteriol., 190, 2615-2618.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii
recombinant expression of the MJ0056 gene in Escherichia coli Methanocaldococcus jannaschii
recombinant expression of the MJ0056 gene in Escherichia coli led to a large increase in the amount of flavin mononucleotide in the Escherichia coli cell extract Methanocaldococcus jannaschii

General Stability

General Stability Organism
10 min at room temperature, followed by dilution in acetate buffer (pH 6.0) Methanocaldococcus jannaschii
the enzyme retains 40% (0.13 nmol/min) of its activity compared to the control (0.32 nmol/min) after treatment with 0.1 M HCl (pH 1.05) for Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1.6 mM, 40% inhibition; 1.6 mM, 61% inhibition Methanocaldococcus jannaschii
Cu2+ 1.6 mM, 3% inhibition; 1.6 mM, 97% inhibition Methanocaldococcus jannaschii
Mg2+ 1.6 mM, 15% inhibition Methanocaldococcus jannaschii
Mn2+ 1.6 mM, 50% inhibition Methanocaldococcus jannaschii
Ni2+ 1.6 mM, 43% inhibition; 1.6 mM, 60% inhibition Methanocaldococcus jannaschii
Zn2+ 1.6 mM, 17% inhibition; 1.6 mM, 83% inhibition Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.159
-
riboflavin
-
Methanocaldococcus jannaschii
0.159
-
riboflavin pH 7.2, 70°C Methanocaldococcus jannaschii
1.8
-
CTP
-
Methanocaldococcus jannaschii
1.8
-
CTP pH 7.2, 70°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme contains 4 to 5 mol of Mg Methanocaldococcus jannaschii
Mg2+ contains 4 to 5 mol of Mg per mol of protein Methanocaldococcus jannaschii
Mg2+ contains 4-5 mol of Mg2+ and 0.0013 mol of Zn2+ per mol of protein Methanocaldococcus jannaschii
additional information absence of a requirement for added metal ion to catalyze the formation of FMN Methanocaldococcus jannaschii
additional information purified recombinant enzyme shows no requirement for added metal ion to catalyze the formation of FMN Methanocaldococcus jannaschii
Zn2+ contains 0.0013 mol of Zn per mol of protein Methanocaldococcus jannaschii
Zn2+ contains 4-5 mol of Mg2+ and 0.0013 mol of Zn2+ per mol of protein Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
1 * 15000, SDS-PAGE Methanocaldococcus jannaschii
15218
-
1 * 15218, electrospray mass spectrometry Methanocaldococcus jannaschii
19700
-
gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q60365
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Methanocaldococcus jannaschii
recombinant enzyme Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + riboflavin 30% of the activity with CTP Methanocaldococcus jannaschii ADP + FMN
-
?
ATP + riboflavin 30% of the activity with ATP Methanocaldococcus jannaschii ADP + riboflavin 5'-phosphate
-
?
CTP + riboflavin most active with CTP as the substrate Methanocaldococcus jannaschii CDP + riboflavin 5'-phosphate
-
?
CTP + riboflavin
-
Methanocaldococcus jannaschii CDP + FMN
-
?
GTP + riboflavin 11% of the activity with ATP Methanocaldococcus jannaschii GDP + riboflavin 5'-phosphate
-
?
GTP + riboflavin 11% of the activity with CTP Methanocaldococcus jannaschii GDP + FMN
-
?

Subunits

Subunits Comment Organism
monomer 1 * 15000, SDS-PAGE Methanocaldococcus jannaschii
monomer 1 * 15218, electrospray mass spectrometry Methanocaldococcus jannaschii
monomer 1 * 19700 Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
MJ0056
-
Methanocaldococcus jannaschii
RibK
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
10 min, stable Methanocaldococcus jannaschii

pH Stability

pH Stability pH Stability Maximum Comment Organism
1
-
10 min at room temperature, 60% loss of activity Methanocaldococcus jannaschii