Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
recombinant expression of the MJ0056 gene in Escherichia coli | Methanocaldococcus jannaschii |
recombinant expression of the MJ0056 gene in Escherichia coli led to a large increase in the amount of flavin mononucleotide in the Escherichia coli cell extract | Methanocaldococcus jannaschii |
General Stability | Organism |
---|---|
10 min at room temperature, followed by dilution in acetate buffer (pH 6.0) | Methanocaldococcus jannaschii |
the enzyme retains 40% (0.13 nmol/min) of its activity compared to the control (0.32 nmol/min) after treatment with 0.1 M HCl (pH 1.05) for | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1.6 mM, 40% inhibition; 1.6 mM, 61% inhibition | Methanocaldococcus jannaschii | |
Cu2+ | 1.6 mM, 3% inhibition; 1.6 mM, 97% inhibition | Methanocaldococcus jannaschii | |
Mg2+ | 1.6 mM, 15% inhibition | Methanocaldococcus jannaschii | |
Mn2+ | 1.6 mM, 50% inhibition | Methanocaldococcus jannaschii | |
Ni2+ | 1.6 mM, 43% inhibition; 1.6 mM, 60% inhibition | Methanocaldococcus jannaschii | |
Zn2+ | 1.6 mM, 17% inhibition; 1.6 mM, 83% inhibition | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.159 | - |
riboflavin | - |
Methanocaldococcus jannaschii | |
0.159 | - |
riboflavin | pH 7.2, 70°C | Methanocaldococcus jannaschii | |
1.8 | - |
CTP | - |
Methanocaldococcus jannaschii | |
1.8 | - |
CTP | pH 7.2, 70°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme contains 4 to 5 mol of Mg | Methanocaldococcus jannaschii | |
Mg2+ | contains 4 to 5 mol of Mg per mol of protein | Methanocaldococcus jannaschii | |
Mg2+ | contains 4-5 mol of Mg2+ and 0.0013 mol of Zn2+ per mol of protein | Methanocaldococcus jannaschii | |
additional information | absence of a requirement for added metal ion to catalyze the formation of FMN | Methanocaldococcus jannaschii | |
additional information | purified recombinant enzyme shows no requirement for added metal ion to catalyze the formation of FMN | Methanocaldococcus jannaschii | |
Zn2+ | contains 0.0013 mol of Zn per mol of protein | Methanocaldococcus jannaschii | |
Zn2+ | contains 4-5 mol of Mg2+ and 0.0013 mol of Zn2+ per mol of protein | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
1 * 15000, SDS-PAGE | Methanocaldococcus jannaschii |
15218 | - |
1 * 15218, electrospray mass spectrometry | Methanocaldococcus jannaschii |
19700 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q60365 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Methanocaldococcus jannaschii |
recombinant enzyme | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + riboflavin | 30% of the activity with CTP | Methanocaldococcus jannaschii | ADP + FMN | - |
? | |
ATP + riboflavin | 30% of the activity with ATP | Methanocaldococcus jannaschii | ADP + riboflavin 5'-phosphate | - |
? | |
CTP + riboflavin | most active with CTP as the substrate | Methanocaldococcus jannaschii | CDP + riboflavin 5'-phosphate | - |
? | |
CTP + riboflavin | - |
Methanocaldococcus jannaschii | CDP + FMN | - |
? | |
GTP + riboflavin | 11% of the activity with ATP | Methanocaldococcus jannaschii | GDP + riboflavin 5'-phosphate | - |
? | |
GTP + riboflavin | 11% of the activity with CTP | Methanocaldococcus jannaschii | GDP + FMN | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 15000, SDS-PAGE | Methanocaldococcus jannaschii |
monomer | 1 * 15218, electrospray mass spectrometry | Methanocaldococcus jannaschii |
monomer | 1 * 19700 | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MJ0056 | - |
Methanocaldococcus jannaschii |
RibK | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
10 min, stable | Methanocaldococcus jannaschii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | - |
10 min at room temperature, 60% loss of activity | Methanocaldococcus jannaschii |