Literature summary for 2.7.1.147 extracted from

Aslam, M.; Takahashi, N.; Matsubara, K.; Imanaka, T.; Kanai, T.; Atomi, H.
Identification of the glucosamine kinase in the chitinolytic pathway of Thermococcus kodakarensis (2018), J. Biosci. Bioeng., 125, 320-326 .

Search for more literature for 2.7.1.147

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Thermococcus kodakarensis

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucosamine	-	Thermococcus kodakarensis
D-glucose	-	Thermococcus kodakarensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	ADP	pH 7.5, 70°C	Thermococcus kodakarensis
0.08	-	ADP	at pH 7.5 and 80°C	Thermococcus kodakarensis
0.33	-	D-glucosamine	pH 7.5, 70°C	Thermococcus kodakarensis
0.33	-	D-glucosamine	at pH 7.5 and 80°C	Thermococcus kodakarensis
0.77	-	D-glucose	pH 7.5, 70°C	Thermococcus kodakarensis
0.77	-	D-glucose	at pH 7.5 and 80°C	Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + D-glucosamine	Thermococcus kodakarensis	the enzyme functions as the glucosamine kinase responsible for the chitin degradation	AMP + D-glucosamine 6-phosphate	-	?
ADP + D-glucosamine	Thermococcus kodakarensis ATCC BAA-918	the enzyme functions as the glucosamine kinase responsible for the chitin degradation	AMP + D-glucosamine 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	-	-	-
Thermococcus kodakarensis	Q5JE39	-	-
Thermococcus kodakarensis ATCC BAA-918	Q5JE39	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermococcus kodakarensis
Resource Q column chromatography and Superdex S200 gel filtration	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + D-glucosamine	-	Thermococcus kodakarensis	AMP + D-glucosamine 6-phosphate	-	?
ADP + D-glucosamine	the enzyme functions as the glucosamine kinase responsible for the chitin degradation	Thermococcus kodakarensis	AMP + D-glucosamine 6-phosphate	-	?
ADP + D-glucosamine	-	Thermococcus kodakarensis ATCC BAA-918	AMP + D-glucosamine 6-phosphate	-	?
ADP + D-glucosamine	the enzyme functions as the glucosamine kinase responsible for the chitin degradation	Thermococcus kodakarensis ATCC BAA-918	AMP + D-glucosamine 6-phosphate	-	?
ADP + D-glucose	-	Thermococcus kodakarensis	AMP + D-glucose 6-phosphate	-	?
ADP + D-glucose	-	Thermococcus kodakarensis ATCC BAA-918	AMP + D-glucose 6-phosphate	-	?
ADP + N-acetyl-D-glucosamine	the enzyme exhibits a low level of activity against N-acetyl-D-glucosamine	Thermococcus kodakarensis	AMP + N-acetyl-D-glucosamine 6-phosphate	-	?
additional information	activity is not observed toward chitobiose and N-,N-diacetylchitobiose	Thermococcus kodakarensis	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 48000, SDS-PAGE	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
GlcN kinase	-	Thermococcus kodakarensis
glucosamine kinase	-	Thermococcus kodakarensis
TK1110	-	Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
120	-	D-glucosamine	pH 7.5, 70°C	Thermococcus kodakarensis
120	-	D-glucosamine	at pH 7.5 and 80°C	Thermococcus kodakarensis
210	-	ADP	pH 7.5, 70°C	Thermococcus kodakarensis
210	-	ADP	at pH 7.5 and 80°C	Thermococcus kodakarensis
248	-	D-glucose	pH 7.5, 70°C	Thermococcus kodakarensis
248	-	D-glucose	at pH 7.5 and 80°C	Thermococcus kodakarensis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
23	-	D-glucose	at pH 7.5 and 80°C	Thermococcus kodakarensis
23	-	D-glucose	pH 7.5, 70°C	Thermococcus kodakarensis
60	-	D-glucosamine	at pH 7.5 and 80°C	Thermococcus kodakarensis
60	-	D-glucosamine	pH 7.5, 70°C	Thermococcus kodakarensis

General Information

General Information	Comment	Organism
malfunction	TK1110 disruption results in almost complete impairment in chitin degradation, and a complete loss of chitin-dependent H2 production	Thermococcus kodakarensis
metabolism	enzyme TK1110 functions as the glucosamine kinase responsible for the chitin degradation in Thermococcus kodakarensis	Thermococcus kodakarensis
physiological function	the enzyme functions as the glucosamine kinase responsible for the chitin degradation	Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
320	-	D-glucose	at pH 7.5 and 80°C	Thermococcus kodakarensis
360	-	D-glucosamine	at pH 7.5 and 80°C	Thermococcus kodakarensis

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Release 2023.1 (January 2023)