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Literature summary for 2.7.1.119 extracted from

  • Li, D.; Tang, N.; Liu, M.; Shen, C.; Hu, Y.; Xia, Y.; Cao, M.
    Using hygromycin phosphotransferase and enhanced green fluorescent protein genes for tracking plastid transformation in rice (Oryza sativa L.) via gold particle bombardment (2016), Nanosci. Nanotechnol. Lett., 8, 409-417 .
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Application

Application Comment Organism
biotechnology use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa) Streptomyces sp.
molecular biology use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa) Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant gene expression in transgenic Oryza sativa from site-specific transformation vector of rice plastid, pCTE04 Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
additional information use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice. A site-specific transformation vector of rice plastid, pCTE04, is constructed for dicistronic expression of the hygromycin phosphotransferase (hpt) and enhanced green fluorescent protein genes (EGFP). Transplastomic rice plants successfully grow up after a gradual selection of hygromycin resistance. Integration of the dicistronic expression cassette into the intergenic region trnI-trnA of plastid genome is verified by Southern blotting analysis. Transplastomic expression of the hpt and EGFP genes is identified by RT-PCR. The stable expression of dicistronic cassette in the rice is confirmed by confocal microscopy Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
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Synonyms

Synonyms Comment Organism
hygromycin phosphotransferase
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 Streptomyces sp.