Literature summary for 2.7.1.11 extracted from

Fernandes, P.; Kinkead, J.; McNae, I.; Bringaud, F.; Michels, P.; Walkinshaw, M.
The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction (2019), Biochem. J., 476, 179-191 .

Search for more literature for 2.7.1.11

Activating Compound

Activating Compound	Comment	Organism	Structure
AMP	0.5 mM, 10fold activation of reaction with D-fructose 1,6-bisphosphate and ADP	Leishmania infantum
AMP	0.5 mM, 2fold activation of reaction with D-fructose 1,6-bisphosphate and ADP	Trypanosoma cruzi
AMP	0.5 mM, 2fold activation of reaction with D-fructose 1,6-bisphosphate and ADP	Trypanosoma brucei brucei

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens
expression in Escherichia coli	Trypanosoma cruzi
expression in Escherichia coli	Trypanosoma brucei brucei
expression in Escherichia coli	Leishmania infantum

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	substrate inhibition above 2 mM; substrate inhibition above 2 mM; substrate inhibition above 2 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	ADP	K0.5: 0.0668 mM, pH 7.4, 25°C, isoenzyme PFK-M	Homo sapiens
additional information	-	ADP	K0.5: 0.3449 mM, pH 7.4, 25°C, isoenzyme PFK-L	Homo sapiens
additional information	-	D-fructose 1,6-bisphosphate	K0.5: 0.7178 mM, pH 7.4, 25°C, isoenzyme PFK-P	Homo sapiens
additional information	-	D-fructose 1,6-bisphosphate	K0.5: 0.804 mM, pH 7.4, 25°C, isoenzyme PFK-M	Homo sapiens
additional information	-	D-fructose 1,6-bisphosphate	K0.5: 1.287 mM, pH 7.4, 25°C	Trypanosoma brucei brucei
additional information	-	ADP	K0.5: 1.382 mM, pH 7.4, 25°C	Trypanosoma brucei brucei
additional information	-	D-fructose 1,6-bisphosphate	K0.5: 1.54, pH 7.4, 25°C	Trypanosoma cruzi
additional information	-	ADP	K0.5: 1.768 mM, pH 7.4, 25°C	Trypanosoma cruzi
additional information	-	D-fructose 1,6-bisphosphate	K0.5: 2.1 mM, pH 7.4, 25°C, isoenzyme PFK-L	Homo sapiens
additional information	-	D-fructose 1,6-bisphosphate	K0.5: 2.495 mM, pH 7.4, 25°C	Leishmania infantum
additional information	-	ADP	K0.5: 3.137 mM, pH 7.4, 25°C	Leishmania infantum

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P08237	-	-
Homo sapiens	P17858	-	-
Homo sapiens	Q01813	-	-
Leishmania infantum	A4I4W5	-	-
Trypanosoma brucei brucei	O15648	-	-
Trypanosoma cruzi	Q4E657	-	-
Trypanosoma cruzi CL Brener	Q4E657	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + D-fructose 1,6-bisphosphate	-	Trypanosoma cruzi	ATP + D-fructose 6-phosphate	-	r
ADP + D-fructose 1,6-bisphosphate	-	Trypanosoma brucei brucei	ATP + D-fructose 6-phosphate	-	r
ADP + D-fructose 1,6-bisphosphate	-	Leishmania infantum	ATP + D-fructose 6-phosphate	-	r
ADP + D-fructose 1,6-bisphosphate	for human cells, the measured K0.5(ADP)0:5 values for the reaction with ADP and D-fructose 1,6-bisphosphate exceeds the cytosolic concentrations of ADP, but as steady-state concentrations of F0.5(D-fructose 1,6-bisphosphate) are lower than the K0.5(D-fructose 1,6-bisphosphate) values, it seems likely that under steady-state conditions the reverse reaction would indeed be inefficient. However, cells can be subjected to extreme conditions of stress and nutrient deprivation and the phosphofructokinase reverse flux may be very different in non-steady state, glucose-poor conditions and Q values of 500 or above can easily be achieved if ATP or glucose-6-phosphate are (transiently) depleted	Homo sapiens	ATP + D-fructose 6-phosphate	-	r
ADP + D-fructose 1,6-bisphosphate	-	Trypanosoma cruzi CL Brener	ATP + D-fructose 6-phosphate	-	r
ATP + D-fructose 6-phosphate	-	Trypanosoma cruzi	ADP + D-fructose 1,6-bisphosphate	-	r
ATP + D-fructose 6-phosphate	-	Trypanosoma brucei brucei	ADP + D-fructose 1,6-bisphosphate	-	r
ATP + D-fructose 6-phosphate	-	Leishmania infantum	ADP + D-fructose 1,6-bisphosphate	-	r
ATP + D-fructose 6-phosphate	all three human PFK isoforms can catalyse the reverse reaction in vitro	Homo sapiens	ADP + D-fructose 1,6-bisphosphate	-	r
ATP + D-fructose 6-phosphate	-	Trypanosoma cruzi CL Brener	ADP + D-fructose 1,6-bisphosphate	-	r

Synonyms

Synonyms	Comment	Organism
ATP-dependent PFK	-	Homo sapiens
ATP-dependent PFK	-	Trypanosoma cruzi
ATP-dependent PFK	-	Trypanosoma brucei brucei
ATP-dependent PFK	-	Leishmania infantum
LmPFK	-	Leishmania infantum
PFK-L	-	Homo sapiens
PFK-M	-	Homo sapiens
PFK-P	-	Homo sapiens
phosphofructokinase	-	Homo sapiens
phosphofructokinase	-	Trypanosoma cruzi
phosphofructokinase	-	Trypanosoma brucei brucei
phosphofructokinase	-	Leishmania infantum
TbPFK	-	Trypanosoma brucei brucei
TcPFK	-	Trypanosoma cruzi

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Release 2023.1 (January 2023)