Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 50 mM, 50% inhibition | Aeropyrum pernix | |
KCl | 100 mM, 50% inhibition | Aeropyrum pernix | |
NaCl | 1 M, 50% inhibition | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
ATP | pH 6.7, 85°C | Aeropyrum pernix | |
0.6 | - |
D-glucose 6-phosphate | pH 6.7, 85°C | Aeropyrum pernix | |
0.68 | - |
D-fructose 6-phosphate | pH 6.7, 85°C | Aeropyrum pernix | |
1 | - |
adenosine | pH 6.7, 85°C | Aeropyrum pernix | |
1 | - |
fructose | pH 6.7, 85°C | Aeropyrum pernix | |
3 | - |
D-ribose 5-phosphate | pH 6.7, 85°C | Aeropyrum pernix | |
5 | - |
D-ribose | pH 6.7, 85°C | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Aeropyrum pernix | |
Mg2+ | required for activity | Aeropyrum pernix | |
Mn2+ | 9% of activity with Mg2+ | Aeropyrum pernix | |
Ni2+ | 15% of activity with Mg2+ | Aeropyrum pernix | |
Zn2+ | 46% of activity with Mg2+ | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33392 | - |
alpha4, x * 33392, deduced from nucleotide sequence | Aeropyrum pernix |
115000 | - |
gel filtration | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Aeropyrum pernix | - |
ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YG89 | hyperthermophilic crenarchaeote | - |
Purification (Comment) | Organism |
---|---|
recombinant PFK, Bio-Scale Q5, gel filtration | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | - |
Aeropyrum pernix | ADP + ? | - |
? | |
ATP + D-fructose 6-phosphate | - |
Aeropyrum pernix | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + fructose | - |
Aeropyrum pernix | ADP + fructose 1-phosphate | - |
? | |
ATP + glucose 6-phosphate | - |
Aeropyrum pernix | ADP + glucose 1,6-bisphosphate | - |
? | |
ATP + ribose | - |
Aeropyrum pernix | ADP + ribose 1-phosphate | - |
? | |
ATP + ribose 5-phosphate | - |
Aeropyrum pernix | ADP + ribose 1,5-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | 76% of activity with ATP | Aeropyrum pernix | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | 76% of activity with ATP | Aeropyrum pernix | IDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, x * 33392, deduced from nucleotide sequence | Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 100 | very low activity at 40°C and 100°C, approx. 50% of maximal activity at 75°C, activation energy: 57 kJ/mol | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
no loss of activity after 2 h | Aeropyrum pernix |
95 | - |
complete loss of activity after 2 h | Aeropyrum pernix |
100 | - |
complete loss of activity after 30 min, 10% and 45% loss of activity after 30 min in the presence of 1 M KCl and NaCl, respectively | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | 60% of maximal activity at pH 5.0 and pH 8.5 | Aeropyrum pernix |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ammonium sulfate | pH 6.7, 85°C | Aeropyrum pernix | |
0.1 | - |
KCl | pH 6.7, 85°C | Aeropyrum pernix | |
1 | - |
NaCl | pH 6.7, 85°C | Aeropyrum pernix |