Cloned (Comment) | Organism |
---|---|
both parent and double mutant (K472A/K473A) constructs are transformed into the BL21 strain of Escherichia coli, overexpression of PFKFB3 causes no change in glucose metabolism but rather a marked increase in cell proliferation | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K472A/K473A | cytoplasmic mutant | Homo sapiens |
R75A/R76A | kinase inactive | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
nucleus | PFKFB3 splice variant 5, the carboxyl terminus is required for this localization | Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Homo sapiens | - |
ADP + beta-D-fructose 2,6-bisphosphate | regulation of glycolysis | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16875 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
MDA-MB-231 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
nuclei isolated from cells transfected with wild type PFKFB3 exhibit 6-phosphofructo-2-kinase activity that is significantly higher than the nuclei obtained from both vector and K472A/K473A-transfected cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Homo sapiens | ADP + beta-D-fructose 2,6-bisphosphate | regulation of glycolysis | ? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-2-kinase | - |
Homo sapiens |
PFKFB3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | ectopic expression of wild type PFKFB3 increases phosphorylation of the cell cycle inhibitor p27, a member of the Kip/Cip family of proteins and a universal inhibitor of cyclin-dependent kinases and the cell cycle, at threonine 187 and decreases total p27 protein levels | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | PFKFB3 enzymes are activated in human cancers | Homo sapiens |
physiological function | PFKFB3 has a role in nuclear signaling | Homo sapiens |