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show all sequences of 2.6.99.3

High-level heterologous production of D-cycloserine by Escherichia coli

Kumagai, T.; Ozawa, T.; Tanimoto, M.; Noda, M.; Matoba, Y.; Sugiyama, M.; Appl. Environ. Microbiol. 81, 7881-7887 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
expression of a D-cycloserine biosynthetic gene cluster consisting of 10 open reading frames (dcsA to dcsJ) from Streptomyces lavendulae ATCC 11924 in Escherichia coli. When L-serine and hydroxyurea, the precursors of D-cycloserine, are incubated together with the Escherichia coli resting cell suspension, the cells produce significant amounts of D-cycloserine (350 microM). To increase the productivity, the dcsJ gene, which might be responsible for the excretion, is connected downstream of the four genes, resulting in production of D-cycloserine at 660 microM. To repress the side catalytic activity of DcsD, i.e. the formation of L-cysteine from O-acetylserine and H2S, Escherichia coli chromosomal cysJ and cysK genes, encoding the sulfite reductase alpha subunit and O-acetylserine sulfhydrylase, respectively, are disrupted. The final strain produces 980 microM D-cycloserine
Streptomyces lavendulae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces lavendulae
D2Z027
-
-
Streptomyces lavendulae ATCC 11924
D2Z027
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae
?
-
-
-
-
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae ATCC 11924
?
-
-
-
-
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
expression of a D-cycloserine biosynthetic gene cluster consisting of 10 open reading frames (dcsA to dcsJ) from Streptomyces lavendulae ATCC 11924 in Escherichia coli. When L-serine and hydroxyurea, the precursors of D-cycloserine, are incubated together with the Escherichia coli resting cell suspension, the cells produce significant amounts of D-cycloserine (350 microM). To increase the productivity, the dcsJ gene, which might be responsible for the excretion, is connected downstream of the four genes, resulting in production of D-cycloserine at 660 microM. To repress the side catalytic activity of DcsD, i.e. the formation of L-cysteine from O-acetylserine and H2S, Escherichia coli chromosomal cysJ and cysK genes, encoding the sulfite reductase alpha subunit and O-acetylserine sulfhydrylase, respectively, are disrupted. The final strain produces 980 microM D-cycloserine
Streptomyces lavendulae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae
?
-
-
-
-
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae ATCC 11924
?
-
-
-
-
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
Other publictions for EC 2.6.99.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737505
Kumagai
High-level heterologous produc ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
Appl. Environ. Microbiol.
81
7881-7887
2015
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738218
Uda
The structural and mutational ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
FEBS J.
282
3929-3944
2015
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721306
Uda
. Establishment of an in vitro ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
Antimicrob. Agents Chemother.
57
2603-2612
2013
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2
721300
Kumagai
Molecular cloning and heterolo ...
Streptomyces lavendulae, Streptomyces lavendulae ATCC 11924
Antimicrob. Agents Chemother.
54
1132-1139
2010
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