Cloned (Comment) | Organism |
---|---|
gene DAP, genotyping | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the dapat (aberrant growth death 2 (agd-2)) mutation of Arabidopsis thaliana leads to metabolic shifts and growth inhibition. Transcriptional and two-dimensional (isoelectric focusing/SDS-PAGE) proteome analyses reveal alterations in the abundance of several transcripts and proteins associated with photosynthesis and photorespiration coupled with a high glycine/serine ratio and increased levels of stress-responsive amino acids. Transcriptome changes induced by the DAPAT mutation, quantitative RT-PCR expression analysis, detailed overview. The leaf proteome is affected by the DAPAT mutation | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-diaminoheptanedioate + 2-oxoglutarate | Arabidopsis thaliana | - |
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O | - |
r | |
LL-2,6-diaminoheptanedioate + 2-oxoglutarate | Arabidopsis thaliana Col-0 | - |
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93ZN9 | - |
- |
Arabidopsis thaliana Col-0 | Q93ZN9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-diaminoheptanedioate + 2-oxoglutarate | - |
Arabidopsis thaliana | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O | - |
r | |
LL-2,6-diaminoheptanedioate + 2-oxoglutarate | - |
Arabidopsis thaliana Col-0 | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
DAP | - |
Arabidopsis thaliana |
LL-DAPAT | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | Arabidopsis thaliana DAP mutant dapat shows reduced activity of the Lys biosynthesis enzyme L,L-diaminopimelate aminotransferase, physiological and metabolic impacts of impaired Lys biosynthesis, the mutation leads to metabolic shifts and growth inhibition, phenotype, overview. No differences in dark respiration between genotypes are observed, but a lower storage and consumption of starch and sugars is observed in dapat plants, also higher protein turnover but no differences in total amino acids during a diurnal cycle in dapat plants. Biochemical alterations rather than stomatal limitations are responsible for the decreased photosynthesis and growth of the dapat mutant, which mimics stress conditions associated with impairments in the Lys biosynthesis pathway | Arabidopsis thaliana |
metabolism | the enzyme is involved in the lysine pathway, overview. Lysine is synthetized in the chloroplast using aspartate as a precursor. Dihydrodipicolinate synthase (DHDPS) is the first enzyme of lysine biosynthesis and it requires pyruvate export from the cytosol to the chloroplast. Under stress conditions, lysine is exported from the chloroplast to mitochondria to be degraded, and electrons are used as a donor for ATP synthesis | Arabidopsis thaliana |