BRENDA - Enzyme Database show
show all sequences of 2.6.1.73

Allylglucosinolate biosynthesis in Brassica carinata

Glover, J.R.; Chapple, C.C.S.; Rothwell, S.; Tober, I.; Ellis, B.E.; Phytochemistry 27, 1345-1348 (1988)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Brassica carinata
involved in the biosynthesis of methionine-derived glucosinolates
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica carinata
-
var. R-4218
-
Purification (Commentary)
Commentary
Organism
var. R-4218, partial
Brassica carinata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Brassica carinata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
187% of activity compared to glyoxylate
636989
Brassica carinata
2-oxoglutarate + L-methionine
-
-
-
?
L-glutamine + glyoxylate
as good as L-methionine
636989
Brassica carinata
2-oxoglutarate + glycine
-
-
-
?
L-histidine + glyoxylate
16% of activity compared to L-methionine
636989
Brassica carinata
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-homoserine + glyoxylate
56% of activity compared to L-methionine
636989
Brassica carinata
4-hydroxy-2-oxobutanoate + glycine
-
-
-
?
L-methionine + 2-oxoglutarate
19% of activity compared to glyoxylate
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
90% of activity compared to glyoxylate
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-methionine
-
-
-
?
L-methionine + glyoxylate
-
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + glycine
-
636989
Brassica carinata
?
L-methionine + oxaloacetate
-
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-aspartate
-
-
-
?
L-methionine + pyruvate
35% of activity compared to glyoxylate
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-alanine
-
-
-
?
L-methionine sulfone + glyoxylate
29% of activity compared to L-methionine
636989
Brassica carinata
4-methylsulfonyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine sulfoxide + glyoxylate
54% of activity compared to L-methionine
636989
Brassica carinata
4-methylsulfinyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine sulfoximine + glyoxylate
35% of activity compared to L-methionine
636989
Brassica carinata
(S-methylsulfonimidoyl)-2-oxobutanoate + glycine
-
-
-
?
additional information
involved in the biosynthesis of methionine-derived glucosinolates
636989
Brassica carinata
?
-
-
-
-
S-methyl-L-cysteine + glyoxylate
49% of activity compared to L-methionine
636989
Brassica carinata
3-methylsulfanyl-2-oxopropanoate + glycine
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Brassica carinata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Brassica carinata
involved in the biosynthesis of methionine-derived glucosinolates
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
var. R-4218, partial
Brassica carinata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Brassica carinata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
187% of activity compared to glyoxylate
636989
Brassica carinata
2-oxoglutarate + L-methionine
-
-
-
?
L-glutamine + glyoxylate
as good as L-methionine
636989
Brassica carinata
2-oxoglutarate + glycine
-
-
-
?
L-histidine + glyoxylate
16% of activity compared to L-methionine
636989
Brassica carinata
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-homoserine + glyoxylate
56% of activity compared to L-methionine
636989
Brassica carinata
4-hydroxy-2-oxobutanoate + glycine
-
-
-
?
L-methionine + 2-oxoglutarate
19% of activity compared to glyoxylate
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
90% of activity compared to glyoxylate
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-methionine
-
-
-
?
L-methionine + glyoxylate
-
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + glycine
-
636989
Brassica carinata
?
L-methionine + oxaloacetate
-
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-aspartate
-
-
-
?
L-methionine + pyruvate
35% of activity compared to glyoxylate
636989
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-alanine
-
-
-
?
L-methionine sulfone + glyoxylate
29% of activity compared to L-methionine
636989
Brassica carinata
4-methylsulfonyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine sulfoxide + glyoxylate
54% of activity compared to L-methionine
636989
Brassica carinata
4-methylsulfinyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine sulfoximine + glyoxylate
35% of activity compared to L-methionine
636989
Brassica carinata
(S-methylsulfonimidoyl)-2-oxobutanoate + glycine
-
-
-
?
additional information
involved in the biosynthesis of methionine-derived glucosinolates
636989
Brassica carinata
?
-
-
-
-
S-methyl-L-cysteine + glyoxylate
49% of activity compared to L-methionine
636989
Brassica carinata
3-methylsulfanyl-2-oxopropanoate + glycine
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Brassica carinata
Other publictions for EC 2.6.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636990
Chapple
Purification and characterizat ...
Brassica carinata, Brassica napus
Plant Physiol.
94
1887-1896
1990
-
-
-
-
-
-
-
-
-
-
4
3
-
2
-
-
2
-
-
2
4
2
12
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
3
-
-
-
2
-
2
4
2
12
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
636989
Glover
-
Allylglucosinolate biosynthesi ...
Brassica carinata
Phytochemistry
27
1345-1348
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
14
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
14
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-