Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | - |
Methanococcus aeolicus | |
L-cysteine | 10 mM | Methanococcus aeolicus | |
methionine | 10 mM, inhibits activity of isoenzyme ArAT-I with 1 mM tyrosine by 23%, isoenzyme ArAT-II is not affected | Methanococcus aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase II | Methanococcus aeolicus | |
0.4 | - |
L-phenylalanine | pH 7.5, 37°C, aromatic aminotransferase I | Methanococcus aeolicus | |
0.4 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase I | Methanococcus aeolicus | |
0.6 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase I | Methanococcus aeolicus | |
0.6 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase II | Methanococcus aeolicus | |
1 | - |
L-tyrosine | pH 7.5, 37°C, aromatic aminotransferase I | Methanococcus aeolicus | |
1 | - |
L-tryptophan | pH 7.5, 37°C, aromatic aminotransferase I | Methanococcus aeolicus | |
1.2 | - |
L-phenylalanine | pH 7.5, 37°C, aromatic aminotransferase II | Methanococcus aeolicus | |
1.8 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase I | Methanococcus aeolicus | |
1.9 | - |
L-tryptophan | pH 7.5, 37°C, aromatic aminotransferase II | Methanococcus aeolicus | |
2.1 | - |
L-tyrosine | pH 7.5, 37°C, aromatic aminotransferase II | Methanococcus aeolicus | |
4.6 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase II | Methanococcus aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
ArAT-II, gel filtration | Methanococcus aeolicus |
150000 | - |
ArAT-I, gel filtration | Methanococcus aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-oxoglutarate | Methanococcus aeolicus | - |
phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | Methanococcus aeolicus | - |
3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | Methanococcus aeolicus | - |
p-hydroxyphenylpyruvate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Methanococcus aeolicus |
partial purification of enzyme forms ArAT-I and ArAT-II | Methanococcus aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-oxoglutarate | - |
Methanococcus aeolicus | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Methanococcus aeolicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Methanococcus aeolicus | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ArAT-I | - |
Methanococcus aeolicus |
aromatic aminotransferase | - |
Methanococcus aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanococcus aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanococcus aeolicus |