BRENDA - Enzyme Database show
show all sequences of 2.6.1.49

Purification and properties of dihydroxyphenylalanine transaminase from guinea pig brain

Fonnum, F.; Larsen, K.; J. Neurochem. 12, 589-598 (1965)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
pyridoxal phosphate
a pyridoxal phosphate protein, Km: 0.016 mM
Cavia porcellus
General Stability
General Stability
Organism
pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation
Cavia porcellus
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetic acid
1 mM, 75% inhibition
Cavia porcellus
KCN
1 mM, 93% inhibition, pyridoxal 5'-phosphate protects
Cavia porcellus
p-chloromercuriphenyl sulfonic acid
0.02 mM, 92% inhibition, addition of 1 mM glutathione protects to 100%
Cavia porcellus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.48
-
2-oxoglutarate
pH 8.2, 37°C
Cavia porcellus
41
-
L-Dopa
pH 8.2, 37°C
Cavia porcellus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cavia porcellus
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, 52°C, calcium phosphate gel, alumina C, Sephadex G-200
Cavia porcellus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0366
-
-
Cavia porcellus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate
i.e. L-dopa
636682
Cavia porcellus
3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate
i.e. DOPP
636682
Cavia porcellus
?
3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate
most effective amino acceptor
636682
Cavia porcellus
3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate
i.e. DOPP
636682
Cavia porcellus
?
3-(3,4-dihydroxyphenyl)-L-alanine + oxaloacetic acid
i.e. L-Dopa, 10% of activity with 2-oxoglutarate
636682
Cavia porcellus
3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate
-
636682
Cavia porcellus
?
L-tyrosine + 2-oxoglutarate
8% of activity with L-Dopa
636682
Cavia porcellus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636682
Cavia porcellus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Cavia porcellus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation
Cavia porcellus
55
-
6 min, pH 7.0, stable
Cavia porcellus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Cavia porcellus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
approx. 35% of maximal activity at pH 7.0, approx. 60% of activity at pH 9.0
Cavia porcellus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
pyridoxal phosphate
a pyridoxal phosphate protein, Km: 0.016 mM
Cavia porcellus
General Stability (protein specific)
General Stability
Organism
pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation
Cavia porcellus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetic acid
1 mM, 75% inhibition
Cavia porcellus
KCN
1 mM, 93% inhibition, pyridoxal 5'-phosphate protects
Cavia porcellus
p-chloromercuriphenyl sulfonic acid
0.02 mM, 92% inhibition, addition of 1 mM glutathione protects to 100%
Cavia porcellus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.48
-
2-oxoglutarate
pH 8.2, 37°C
Cavia porcellus
41
-
L-Dopa
pH 8.2, 37°C
Cavia porcellus
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, 52°C, calcium phosphate gel, alumina C, Sephadex G-200
Cavia porcellus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0366
-
-
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate
i.e. L-dopa
636682
Cavia porcellus
3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate
i.e. DOPP
636682
Cavia porcellus
?
3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate
most effective amino acceptor
636682
Cavia porcellus
3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate
i.e. DOPP
636682
Cavia porcellus
?
3-(3,4-dihydroxyphenyl)-L-alanine + oxaloacetic acid
i.e. L-Dopa, 10% of activity with 2-oxoglutarate
636682
Cavia porcellus
3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate
-
636682
Cavia porcellus
?
L-tyrosine + 2-oxoglutarate
8% of activity with L-Dopa
636682
Cavia porcellus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636682
Cavia porcellus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Cavia porcellus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation
Cavia porcellus
55
-
6 min, pH 7.0, stable
Cavia porcellus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Cavia porcellus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
approx. 35% of maximal activity at pH 7.0, approx. 60% of activity at pH 9.0
Cavia porcellus
Other publictions for EC 2.6.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673140
Wu
D-dopa is unidirectionally con ...
Rattus norvegicus
Clin. Exp. Pharmacol. Physiol.
33
1042-1046
2006
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1
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-
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5
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1
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1
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1
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1
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636683
Waterhouse
Inhibition of human and rat he ...
Homo sapiens, Rattus norvegicus
Mol. Pharmacol.
15
108-114
1979
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5
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2
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2
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6
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5
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2
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6
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636684
Nagasaki
-
DOPA production with Enterobac ...
Enterobacter cloacae, Enterobacter cloacae NB 320
Agric. Biol. Chem.
39
363-369
1975
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2
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6
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1
1
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2
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1
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6
-
1
1
2
-
2
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1
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-
-
-
-
-
636685
Nagasaki
-
Studies on DOPA transaminase o ...
Alcaligenes faecalis, Alcaligenes faecalis IAM 1015
Agric. Biol. Chem.
37
1701-1706
1973
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6
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2
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6
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1
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3
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2
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6
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6
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1
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3
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2
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-
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-
636682
Fonnum
Purification and properties of ...
Cavia porcellus
J. Neurochem.
12
589-598
1965
1
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1
3
2
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2
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1
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1
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4
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1
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1
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