KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2-Oxoisohexanoate | with L-tryptophan, pH 8.0, 30°C | Pseudomonas sp. | |
0.08 | - |
2-Oxohexanoate | with tryptophan, pH 8.0, 30°C | Pseudomonas sp. | |
0.11 | - |
2-oxoisopentanoate | with L-tryptophan, pH 8.0, 30°C | Pseudomonas sp. | |
0.12 | - |
2-oxopentanoate | with L-tryptophan, pH 8.0, 30°C | Pseudomonas sp. | |
0.2 | - |
Indolepyruvate | with L-leucine, pH 8.0, 30°C | Pseudomonas sp. | |
0.4 | - |
L-leucine | with phenylpyruvate, pH 8.0, 30°C | Pseudomonas sp. | |
0.47 | - |
L-isoleucine | with phenylpyruvate, pH 8.0, 30°C | Pseudomonas sp. | |
0.8 | - |
L-phenylalanine | with 2-oxoisohexanoate, pH 8.0, 30°C | Pseudomonas sp. | |
1 | - |
L-tyrosine | with 2-oxoisohexanoate, pH 8.0, 30°C | Pseudomonas sp. | |
1.4 | - |
2-oxobutanoate | with L-tryptophan, pH 8.0, 30°C | Pseudomonas sp. | |
1.4 | - |
L-tryptophan | with 2-oxoisohexanoate, pH 8.0, 30°C | Pseudomonas sp. | |
3.4 | - |
L-norleucine | with phenylpyruvate, pH 8.0, 30°C | Pseudomonas sp. | |
4.5 | - |
L-valine | with phenylpyruvate, pH 8.0, 30°C | Pseudomonas sp. | |
4.5 | - |
L-norvaline | with phenylpyruvate, pH 8.0, 30°C | Pseudomonas sp. | |
12 | - |
pyruvate | with L-tryptophan, pH 8.0, 30°C | Pseudomonas sp. | |
23.8 | - |
L-histidine | with 2-oxoisohexanoate, pH 8.0, 30°C | Pseudomonas sp. | |
30 | - |
L-2-aminobutanoate | with phenylpyruvate, pH 8.0, 30°C | Pseudomonas sp. | |
250 | - |
L-alanine | with 2-oxoisohexanoate, pH 8.0, 30°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
sedimentation equilibrium analysis | Pseudomonas sp. |
110000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
76 | - |
purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutanoate + phenylpyruvate | - |
Pseudomonas sp. | L-phenylalanine + 2-oxobutanoate | - |
? | |
L-histidine + 2-oxoisohexanoate | - |
Pseudomonas sp. | ? | - |
? | |
L-isoleucine + phenylpyruvate | - |
Pseudomonas sp. | L-phenylalanine + 3-methyl-2-oxopentanoate | - |
? | |
L-phenylalanine + 2-oxobutanoate | low activity | Pseudomonas sp. | phenylpyruvate + 2-aminobutanoate | - |
r | |
L-phenylalanine + 2-oxohexanoate | - |
Pseudomonas sp. | phenylpyruvate + L-norleucine | - |
r | |
L-phenylalanine + 2-oxoisohexanoate | i.e alpha-ketoisocaproate, highest activity as amino acceptor | Pseudomonas sp. | phenylpyruvate + L-leucine | - |
r | |
L-phenylalanine + 2-oxoisopentanoate | - |
Pseudomonas sp. | phenylpyruvate + L-valine | - |
r | |
L-phenylalanine + pyruvate | 2% of the activity with alpha-ketoisocaproate | Pseudomonas sp. | phenylpyruvate + L-alanine | - |
r | |
L-tryptophan + 2-oxobutanoate | weak activity | Pseudomonas sp. | 3-indole-2-oxopropanoate + 2-aminobutanoate | - |
r | |
L-tryptophan + 2-oxoglutarate | no activity | Pseudomonas sp. | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tryptophan + 2-oxohexanoate | - |
Pseudomonas sp. | 3-indole-2-oxopropanoate + L-norleucine | - |
r | |
L-tryptophan + 2-oxoisohexanoate | - |
Pseudomonas sp. | 3-indole-2-oxopropanoate + L-leucine | - |
r | |
L-tryptophan + 2-oxoisopentanoate | i.e. L-Trp + dimethylpyruvate | Pseudomonas sp. | 3-indole-2-oxopropanoate + L-valine | - |
r | |
L-tryptophan + 2-oxopentanoate | - |
Pseudomonas sp. | 3-indole-2-oxopropanoate + norvaline | - |
r | |
L-tryptophan + phenylpyruvate | - |
Pseudomonas sp. | 3-indole-2-oxopropanoate + L-phenylalanine | i.e. indolepyruvate + L-Phe | r | |
L-tryptophan + pyruvate | 2% of the activity with alpha-ketoisocaproate | Pseudomonas sp. | 3-indole-2-oxopropanoate + L-alanine | - |
r | |
additional information | no activity with glutamate, 2-oxoglutarate | Pseudomonas sp. | ? | - |
? | |
phenylpyruvate + DL-5-hydroxytryptophan | - |
Pseudomonas sp. | phenylalanine + 3-(5-hydroxyindole)-2-oxopropanoate | - |
r | |
phenylpyruvate + DL-isoleucine | - |
Pseudomonas sp. | phenylalanine + 3-methyl-2-oxopentanoate | - |
r | |
phenylpyruvate + DL-leucine | - |
Pseudomonas sp. | phenylalanine + 4-methyl-2-oxopentanoate | - |
r | |
phenylpyruvate + DL-methionine | low activity | Pseudomonas sp. | phenylalanine + 4-methylsulfanyl-2-oxobutanoate | - |
r | |
phenylpyruvate + DL-valine | - |
Pseudomonas sp. | phenylalanine + 3-methyl-2-oxobutanoate | - |
r | |
phenylpyruvate + L-2-iminobutanoate | - |
Pseudomonas sp. | phenylalanine + ? | - |
r | |
phenylpyruvate + L-citrulline | low activity | Pseudomonas sp. | phenylalanine + 2-oxo-5-ureido-pentanoate | - |
r | |
phenylpyruvate + L-norleucine | - |
Pseudomonas sp. | phenylalanine + 2-oxohexanoate | - |
r | |
phenylpyruvate + L-tyrosine | - |
Pseudomonas sp. | phenylalanine + 3-(4-hydroxyphenyl)-2-oxopropanoate | - |
r | |
phenylpyruvate + norvaline | - |
Pseudomonas sp. | phenylalanine + 2-oxopentanoate | - |
r | |
phenylpyruvate + phenylglycine | - |
Pseudomonas sp. | phenylalanine + phenylglyoxylate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas sp. |