BRENDA - Enzyme Database show
show all sequences of 2.6.1.28

L-tryptophan-alpha-ketoisocaproate aminotransferase from Pseudomonas sp.

Koide, Y.; Honma, M.; Shimomura, T.; Agric. Biol. Chem. 44, 2013-2019 (1980)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
2-Oxoisohexanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.08
-
2-Oxohexanoate
with tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.11
-
2-oxoisopentanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.12
-
2-Oxopentanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.2
-
Indolepyruvate
with L-leucine, pH 8.0, 30C
Pseudomonas sp.
0.4
-
L-leucine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
0.47
-
L-isoleucine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
0.8
-
L-phenylalanine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
1
-
L-tyrosine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
1.4
-
2-oxobutanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
1.4
-
L-tryptophan
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
3.4
-
L-norleucine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
4.5
-
L-norvaline
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
4.5
-
L-valine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
12
-
pyruvate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
23.8
-
L-histidine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
30
-
L-2-aminobutanoate
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
250
-
L-alanine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
sedimentation equilibrium analysis
Pseudomonas sp.
110000
-
gel filtration
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
76
-
purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine + 2-oxobutanoate
low activity
639949
Pseudomonas sp.
phenylpyruvate + 2-aminobutanoate
-
-
-
r
L-phenylalanine + 2-oxohexanoate
-
639949
Pseudomonas sp.
phenylpyruvate + L-norleucine
-
-
-
r
L-phenylalanine + 2-oxoisohexanoate
i.e alpha-ketoisocaproate, highest activity as amino acceptor
639949
Pseudomonas sp.
phenylpyruvate + L-leucine
-
-
-
r
L-phenylalanine + 2-oxoisopentanoate
-
639949
Pseudomonas sp.
phenylpyruvate + L-valine
-
-
-
r
L-phenylalanine + pyruvate
2% of the activity with alpha-ketoisocaproate
639949
Pseudomonas sp.
phenylpyruvate + L-alanine
-
-
-
r
L-tryptophan + 2-oxobutanoate
weak activity
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + 2-aminobutanoate
-
-
-
r
L-tryptophan + 2-oxoglutarate
no activity
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-glutamate
-
-
-
-
L-tryptophan + 2-oxohexanoate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-norleucine
-
-
-
r
L-tryptophan + 2-oxoisohexanoate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-leucine
-
-
-
r
L-tryptophan + 2-oxoisopentanoate
i.e. L-Trp + dimethylpyruvate
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-valine
-
-
-
r
L-tryptophan + 2-oxopentanoate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + norvaline
-
-
-
r
L-tryptophan + phenylpyruvate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-phenylalanine
i.e. indolepyruvate + L-Phe
639949
Pseudomonas sp.
r
L-tryptophan + pyruvate
2% of the activity with alpha-ketoisocaproate
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-alanine
-
-
-
r
additional information
no activity with glutamate, 2-oxoglutarate
639949
Pseudomonas sp.
?
-
-
-
-
phenylpyruvate + DL-5-hydroxytryptophan
-
639949
Pseudomonas sp.
phenylalanine + 3-(5-hydroxyindole)-2-oxopropanoate
-
-
-
r
phenylpyruvate + DL-isoleucine
-
639949
Pseudomonas sp.
phenylalanine + 3-methyl-2-oxopentanoate
-
-
-
r
phenylpyruvate + DL-leucine
-
639949
Pseudomonas sp.
phenylalanine + 4-methyl-2-oxopentanoate
-
-
-
r
phenylpyruvate + DL-methionine
low activity
639949
Pseudomonas sp.
phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
-
-
r
phenylpyruvate + DL-valine
-
639949
Pseudomonas sp.
phenylalanine + 3-methyl-2-oxobutanoate
-
-
-
r
phenylpyruvate + L-2-iminobutanoate
-
639949
Pseudomonas sp.
phenylalanine + ?
-
-
-
r
phenylpyruvate + L-citrulline
low activity
639949
Pseudomonas sp.
phenylalanine + 2-oxo-5-ureido-pentanoate
-
-
-
r
phenylpyruvate + L-norleucine
-
639949
Pseudomonas sp.
phenylalanine + 2-oxohexanoate
-
-
-
r
phenylpyruvate + L-tyrosine
-
639949
Pseudomonas sp.
phenylalanine + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
-
-
r
phenylpyruvate + norvaline
-
639949
Pseudomonas sp.
phenylalanine + 2-oxopentanoate
-
-
-
r
phenylpyruvate + phenylglycine
-
639949
Pseudomonas sp.
phenylalanine + phenylglyoxylate
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
2-Oxoisohexanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.08
-
2-Oxohexanoate
with tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.11
-
2-oxoisopentanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.12
-
2-Oxopentanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
0.2
-
Indolepyruvate
with L-leucine, pH 8.0, 30C
Pseudomonas sp.
0.4
-
L-leucine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
0.47
-
L-isoleucine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
0.8
-
L-phenylalanine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
1
-
L-tyrosine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
1.4
-
2-oxobutanoate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
1.4
-
L-tryptophan
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
3.4
-
L-norleucine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
4.5
-
L-norvaline
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
4.5
-
L-valine
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
12
-
pyruvate
with L-tryptophan, pH 8.0, 30C
Pseudomonas sp.
23.8
-
L-histidine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
30
-
L-2-aminobutanoate
with phenylpyruvate, pH 8.0, 30C
Pseudomonas sp.
250
-
L-alanine
with 2-oxoisohexanoate, pH 8.0, 30C
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
sedimentation equilibrium analysis
Pseudomonas sp.
110000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
76
-
purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine + 2-oxobutanoate
low activity
639949
Pseudomonas sp.
phenylpyruvate + 2-aminobutanoate
-
-
-
r
L-phenylalanine + 2-oxohexanoate
-
639949
Pseudomonas sp.
phenylpyruvate + L-norleucine
-
-
-
r
L-phenylalanine + 2-oxoisohexanoate
i.e alpha-ketoisocaproate, highest activity as amino acceptor
639949
Pseudomonas sp.
phenylpyruvate + L-leucine
-
-
-
r
L-phenylalanine + 2-oxoisopentanoate
-
639949
Pseudomonas sp.
phenylpyruvate + L-valine
-
-
-
r
L-phenylalanine + pyruvate
2% of the activity with alpha-ketoisocaproate
639949
Pseudomonas sp.
phenylpyruvate + L-alanine
-
-
-
r
L-tryptophan + 2-oxobutanoate
weak activity
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + 2-aminobutanoate
-
-
-
r
L-tryptophan + 2-oxoglutarate
no activity
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-glutamate
-
-
-
-
L-tryptophan + 2-oxohexanoate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-norleucine
-
-
-
r
L-tryptophan + 2-oxoisohexanoate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-leucine
-
-
-
r
L-tryptophan + 2-oxoisopentanoate
i.e. L-Trp + dimethylpyruvate
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-valine
-
-
-
r
L-tryptophan + 2-oxopentanoate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + norvaline
-
-
-
r
L-tryptophan + phenylpyruvate
-
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-phenylalanine
i.e. indolepyruvate + L-Phe
639949
Pseudomonas sp.
r
L-tryptophan + pyruvate
2% of the activity with alpha-ketoisocaproate
639949
Pseudomonas sp.
3-indole-2-oxopropanoate + L-alanine
-
-
-
r
additional information
no activity with glutamate, 2-oxoglutarate
639949
Pseudomonas sp.
?
-
-
-
-
phenylpyruvate + DL-5-hydroxytryptophan
-
639949
Pseudomonas sp.
phenylalanine + 3-(5-hydroxyindole)-2-oxopropanoate
-
-
-
r
phenylpyruvate + DL-isoleucine
-
639949
Pseudomonas sp.
phenylalanine + 3-methyl-2-oxopentanoate
-
-
-
r
phenylpyruvate + DL-leucine
-
639949
Pseudomonas sp.
phenylalanine + 4-methyl-2-oxopentanoate
-
-
-
r
phenylpyruvate + DL-methionine
low activity
639949
Pseudomonas sp.
phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
-
-
r
phenylpyruvate + DL-valine
-
639949
Pseudomonas sp.
phenylalanine + 3-methyl-2-oxobutanoate
-
-
-
r
phenylpyruvate + L-2-iminobutanoate
-
639949
Pseudomonas sp.
phenylalanine + ?
-
-
-
r
phenylpyruvate + L-citrulline
low activity
639949
Pseudomonas sp.
phenylalanine + 2-oxo-5-ureido-pentanoate
-
-
-
r
phenylpyruvate + L-norleucine
-
639949
Pseudomonas sp.
phenylalanine + 2-oxohexanoate
-
-
-
r
phenylpyruvate + L-tyrosine
-
639949
Pseudomonas sp.
phenylalanine + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
-
-
r
phenylpyruvate + norvaline
-
639949
Pseudomonas sp.
phenylalanine + 2-oxopentanoate
-
-
-
r
phenylpyruvate + phenylglycine
-
639949
Pseudomonas sp.
phenylalanine + phenylglyoxylate
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pseudomonas sp.
Other publictions for EC 2.6.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686048
Schneider
A one-pot chemoenzymatic synth ...
Aspergillus nidulans
Chem. Biol.
14
635-644
2007
-
1
1
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
689126
Balibar
Terrequinone A biosynthesis th ...
Aspergillus nidulans
Nat. Chem. Biol.
3
584-592
2007
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
639949
Koide
-
L-tryptophan-alpha-ketoisocapr ...
Pseudomonas sp.
Agric. Biol. Chem.
44
2013-2019
1980
-
-
-
-
-
-
-
18
-
-
2
-
-
1
-
-
1
-
-
-
1
-
25
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
2
-
-
-
-
1
-
-
1
-
25
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639948
Sukanya
Aminotransferases of Agrobacte ...
Agrobacterium tumefaciens
Biochem. J.
92
594-598
1964
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
1
-
-
-
1
2
12
-
1
-
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
2
12
-
1
-
3
-
1
1
-
-
-
-
-
-
-
-