General Stability | Organism |
---|---|
activity of the purified enzyme is preserved by addition of pyridoxal 5'-phosphate | Rattus norvegicus |
pyridoxal 5'-phosphate stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutanoate | - |
Rattus norvegicus | |
2-oxoglutarate | alpha-keto acid acceptor, inhibitor at high concentrations | Rattus norvegicus | |
2-Oxohexanoate | - |
Rattus norvegicus | |
2-oxoisopentanoate | - |
Rattus norvegicus | |
4-aminobutanoate | - |
Rattus norvegicus | |
5-Aminopentanoate | - |
Rattus norvegicus | |
cadaverine | - |
Rattus norvegicus | |
DL-norvaline | - |
Rattus norvegicus | |
glyoxylate | alpha-keto acid acceptor, inhibitor at high concentrations | Rattus norvegicus | |
L-canavanine | complete inhibition at 0.025 M | Rattus norvegicus | |
L-leucine | - |
Rattus norvegicus | |
L-valine | - |
Rattus norvegicus | |
ornithine | at high concentrations | Rattus norvegicus | |
putrescine | - |
Rattus norvegicus | |
pyruvate | alpha-keto acid acceptor, inhibitor at high concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
2-oxoglutarate | pH 7.1, 37°C | Rattus norvegicus | |
1.7 | - |
ornithine | with pyruvate as cosubstrate, pH 7.1, 37°C | Rattus norvegicus | |
2.8 | - |
ornithine | with 2-oxoglutarate as cosubstrate, pH 7.1, 37°C | Rattus norvegicus | |
2.9 | - |
ornithine | with glyoxylate as cosubstrate, pH 7.1, 37°C | Rattus norvegicus | |
6.7 | - |
glyoxylate | pH 7.1, 37°C | Rattus norvegicus | |
22 | - |
pyruvate | pH 7.1, 37°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | Rattus norvegicus | metabolism of proline to ornithine | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-15°C, rat liver homogenate in sucrose or phosphate solution, stable for some weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | D-ornithine is not accepted as substrate | Rattus norvegicus | DELTA1-pyrroline-5-carboxylate + L-glutamate | equilibrium constant: 71 | ? | |
L-ornithine + 2-oxoglutarate | metabolism of proline to ornithine | Rattus norvegicus | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + glyoxylate | - |
Rattus norvegicus | DELTA1-pyrroline-5-carboxylate + glycine | - |
? | |
L-ornithine + pyruvate | - |
Rattus norvegicus | DELTA1-pyrroline-5-carboxylate + L-alanine | - |
? | |
additional information | inactive as amino group acceptor: 2-oxoisohexanoate | Rattus norvegicus | ? | - |
? | |
additional information | inactive as amino group acceptor: 2-oxoisopentanoate | Rattus norvegicus | ? | - |
? | |
additional information | inactive as amino group acceptor: oxaloacetate | Rattus norvegicus | ? | - |
? | |
additional information | specific for ornithine | Rattus norvegicus | ? | - |
? | |
additional information | inactive as amino group acceptor: 2-oxobutanoate | Rattus norvegicus | ? | - |
? | |
additional information | inactive as amino group acceptor: 2-oxopentanoate | Rattus norvegicus | ? | - |
? | |
additional information | inactive as amino group acceptor: 2-oxohexanoate | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |