Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | preincubation with pyridoxal phosphate leads to an increase in the enzyme activity and the protection of the activity against heat inactivation at 40-60 and 65-80°C, respectively | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-methyl-2-benzothiazolone hydrazone | - |
Pseudomonas sp. | |
aminoxy acetate | - |
Pseudomonas sp. | |
D-cycloserine | - |
Pseudomonas sp. | |
hydroxylamine | - |
Pseudomonas sp. | |
phenylhydrazine | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00625 | - |
pyruvate | pH 10.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0769 | - |
putrescine | pH 10.0, temperature not specified in the publication | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diaminoethane + pyruvate | Pseudomonas sp. | 38% of the activity with beta-alanine | aminoethane + alanine | - |
? | |
1,2-diaminoethane + pyruvate | Pseudomonas sp. F-126 | 38% of the activity with beta-alanine | aminoethane + alanine | - |
? | |
1,6-diaminohexane + pyruvate | Pseudomonas sp. | 87% of the activity with beta-alanine | 1-aminohexane + alanine | - |
? | |
1,6-diaminohexane + pyruvate | Pseudomonas sp. F-126 | 87% of the activity with beta-alanine | 1-aminohexane + alanine | - |
? | |
1,7-diaminoheptane + pyruvate | Pseudomonas sp. | 92% of the activity with beta-alanine | 1-aminoheptane + alanine | - |
? | |
1,7-diaminoheptane + pyruvate | Pseudomonas sp. F-126 | 92% of the activity with beta-alanine | 1-aminoheptane + alanine | - |
? | |
1,8-diaminooctane + pyruvate | Pseudomonas sp. | 55% of the activity with beta-alanine | 1-aminooctane + alanine | - |
? | |
1,8-diaminooctane + pyruvate | Pseudomonas sp. F-126 | 55% of the activity with beta-alanine | 1-aminooctane + alanine | - |
? | |
beta-alanine + pyruvate | Pseudomonas sp. | best substrate | 3-oxopropionate + alanine | - |
? | |
cadaverine + pyruvate | Pseudomonas sp. | 12% of the activity with beta-alanine | 1-aminopentane + alanine | - |
? | |
additional information | Pseudomonas sp. | one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane | ? | - |
? | |
additional information | Pseudomonas sp. F-126 | one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane | ? | - |
? | |
putrescine + pyruvate | Pseudomonas sp. | 55% of the activity with beta-alanine | 4-aminobutanal + alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. F-126 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diaminoethane + pyruvate | 38% of the activity with beta-alanine | Pseudomonas sp. | aminoethane + alanine | - |
? | |
1,2-diaminoethane + pyruvate | 38% of the activity with beta-alanine | Pseudomonas sp. F-126 | aminoethane + alanine | - |
? | |
1,6-diaminohexane + pyruvate | 87% of the activity with beta-alanine | Pseudomonas sp. | 1-aminohexane + alanine | - |
? | |
1,6-diaminohexane + pyruvate | 87% of the activity with beta-alanine | Pseudomonas sp. F-126 | 1-aminohexane + alanine | - |
? | |
1,7-diaminoheptane + pyruvate | 92% of the activity with beta-alanine | Pseudomonas sp. | 1-aminoheptane + alanine | - |
? | |
1,7-diaminoheptane + pyruvate | 92% of the activity with beta-alanine | Pseudomonas sp. F-126 | 1-aminoheptane + alanine | - |
? | |
1,8-diaminooctane + pyruvate | 55% of the activity with beta-alanine | Pseudomonas sp. | 1-aminooctane + alanine | - |
? | |
1,8-diaminooctane + pyruvate | 55% of the activity with beta-alanine | Pseudomonas sp. F-126 | 1-aminooctane + alanine | - |
? | |
beta-alanine + pyruvate | best substrate | Pseudomonas sp. | 3-oxopropionate + alanine | - |
? | |
cadaverine + pyruvate | 12% of the activity with beta-alanine | Pseudomonas sp. | 1-aminopentane + alanine | - |
? | |
additional information | one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane | Pseudomonas sp. | ? | - |
? | |
additional information | one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane | Pseudomonas sp. F-126 | ? | - |
? | |
putrescine + pyruvate | 55% of the activity with beta-alanine | Pseudomonas sp. | 4-aminobutanal + alanine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | in presence of pyridoxal 5'-phosphate, inactivation is delayed | Pseudomonas sp. |
70 | - |
rapid inactivation above | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Pseudomonas sp. |
10.5 | 11 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | stable within | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas sp. |
Organism | Comment | Expression |
---|---|---|
Pseudomonas sp. | expression is induced in presence of beta-alanine | up |