Application | Comment | Organism |
---|---|---|
synthesis | mutant enzyme R292E/L18H can use L-lysine as inexpensive amino donor for the production of L-homophenylalanine. The low solubility of product L-homophenylalanine and spontaneous cyclization of 2-keto-6-aminocaproate drive the reaction completely towards production of L-homophenylalanine | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R292E/L18H | 12.9fold increase in specific activity towards L-Lys and 2-oxo-4-phenylbutanoic acid | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
2-oxo-4-phenyl-butanoic acid | wild-type enzyme | Escherichia coli | |
0.86 | - |
2-oxo-4-phenyl-butanoic acid | mutant enzyme R292E/L18H | Escherichia coli | |
105 | - |
L-Lys | mutant enzyme R292E/L18H | Escherichia coli | |
189 | - |
L-Lys | wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Lys + 2-oxo-4-phenyl-butanoic acid | mutant enzyme R292E/L18H shows a 12.9fold increase in specific activity towards L-Lys and 2-oxo-4-phenylbutanoic acid | Escherichia coli | L-homophenylalanine + 2-keto-6-aminocaproate | 2-keto-6-aminocaproate is cyclized nonenzymatically to form DELTA1-piperideine 2-carboxylic acid in the reaction mixture. The low solubility of L-homophenylalanine and spontaneous cyclization of 2-keto-6-aminocaproate drive the reaction completely towards production of L-homophenylalanine | ? |
Synonyms | Comment | Organism |
---|---|---|
AAT | - |
Escherichia coli |
aspartate aminotransferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.305 | - |
L-Lys | wild-type enzyme | Escherichia coli | |
0.35 | - |
2-oxo-4-phenyl-butanoic acid | wild-type enzyme | Escherichia coli | |
0.38 | - |
2-oxo-4-phenyl-butanoic acid | mutant enzyme R292E/L18H | Escherichia coli | |
5.55 | - |
L-Lys | mutant enzyme R292E/L18H | Escherichia coli |