General Stability | Organism |
---|---|
complete loss of activity after freezing and thawing | Lupinus angustifolius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | product and substrate inhibition | Lupinus angustifolius | |
L-glutamate | product inhibition, noncompetitive to 2-oxoglutarate and competitive against aspartate | Lupinus angustifolius | |
oxaloacetate | isozymes AAT-P1 and AAT-P2; substrate inhibition | Lupinus angustifolius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxaloacetate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
0.1 | - |
oxaloacetate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
0.2 | - |
2-oxoglutarate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
0.26 | - |
2-oxoglutarate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
2.2 | - |
L-aspartate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius | |
2.6 | - |
L-aspartate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
12 | - |
L-glutamate | isozyme AAT-P2, pH 7.6, 25°C | Lupinus angustifolius | |
22 | - |
L-glutamate | isozyme AAT-P1, pH 7.6, 25°C | Lupinus angustifolius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Lupinus angustifolius | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
isozyme AAT-P2, gel filtration | Lupinus angustifolius |
105000 | - |
gel filtration, isozyme AAT-P2 | Lupinus angustifolius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Lupinus angustifolius | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus angustifolius | - |
2 cytosolic isozymes: AAT-P1 and AAT-P2 | - |
Lupinus angustifolius | - |
cv. Uniharvest | - |
Purification (Comment) | Organism |
---|---|
isoenzymes: AAT-P1 and AAT-P2, partial | Lupinus angustifolius |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Lupinus angustifolius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | - |
Lupinus angustifolius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
partially purified enzyme | Lupinus angustifolius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Lupinus angustifolius | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | absolute specificity for aspartate and 2-oxoglutarate and for glutamate and oxaloacetate | Lupinus angustifolius | oxaloacetate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | AAT-P2 und AAT-P2 | Lupinus angustifolius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Lupinus angustifolius |
8 | 8.9 | - |
Lupinus angustifolius |