General Stability | Organism |
---|---|
2-oxoglutarate does not protect against heat inactivation | Chlamydomonas reinhardtii |
addition of glycerol, DTT and pyridoxal 5'-phosphate to buffers, required for stabilization during purification | Chlamydomonas reinhardtii |
aspartate, 10 mM, or pyridoxal 5'-phosphate, 0.2 mM protects against heat inactivation | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
Chlamydomonas reinhardtii | |
L-ascorbate | - |
Chlamydomonas reinhardtii | |
Methylacetimidate | complete inhibition at 50 mM | Chlamydomonas reinhardtii | |
additional information | no substrate inhibition | Chlamydomonas reinhardtii | |
p-hydroxymercuribenzoate | - |
Chlamydomonas reinhardtii | |
Thiosemicarbazide | - |
Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
oxaloacetate | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
0.55 | - |
2-oxoglutarate | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
2.53 | - |
L-aspartate | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
3.88 | - |
L-glutamate | pH 7.8, 25°C | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
138000 | - |
gel filtration | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Chlamydomonas reinhardtii | - |
oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | Chlamydomonas reinhardtii 6145c | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Chlamydomonas reinhardtii 6145c | - |
- |
- |
Purification (Comment) | Organism |
---|---|
400fold | Chlamydomonas reinhardtii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
purified enzyme | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Chlamydomonas reinhardtii | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Chlamydomonas reinhardtii 6145c | oxaloacetate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii 6145c | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | low activity | Chlamydomonas reinhardtii 6145c | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
additional information | substrate specificity | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | substrate specificity | Chlamydomonas reinhardtii 6145c | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 65000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Chlamydomonas reinhardtii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, more than 90% loss of activity | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 2 mol of pyridoxal phosphate per mol of enzyme | Chlamydomonas reinhardtii | |
pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Chlamydomonas reinhardtii |