Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Schizosaccharomyces pombe |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour diffusion method, crystallizes in space group C222(1). The crystals diffract to a resolution of 2.4 A | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
W27F | the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Schizosaccharomyces pombe |
W27G | the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Schizosaccharomyces pombe |
W27H | the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Schizosaccharomyces pombe |
W27I | the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Schizosaccharomyces pombe |
W27S | the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Schizosaccharomyces pombe |
W27Y | the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
riboflavin | - |
Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme W27F | Schizosaccharomyces pombe | |
0.003 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme W27Y | Schizosaccharomyces pombe | |
0.005 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, wild-type enzyme | Schizosaccharomyces pombe | |
0.065 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme W27F | Schizosaccharomyces pombe | |
0.067 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, wild-type enzyme | Schizosaccharomyces pombe | |
0.086 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme W27Y | Schizosaccharomyces pombe | |
0.137 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme W27I | Schizosaccharomyces pombe | |
0.145 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme W27H | Schizosaccharomyces pombe | |
0.168 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme W27G | Schizosaccharomyces pombe | |
0.187 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C, mutant enzyme W27S | Schizosaccharomyces pombe | |
0.23 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme W27I | Schizosaccharomyces pombe | |
0.4 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme W27H | Schizosaccharomyces pombe | |
0.43 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme W27G | Schizosaccharomyces pombe | |
0.46 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C, mutant enzyme W27S | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17188 | - |
5 * 17188, calculated from sequence | Schizosaccharomyces pombe |
17189 | - |
5 * 17189, electrospray MS | Schizosaccharomyces pombe |
87000 | - |
sedimentation equilibrium centrifugation | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Schizosaccharomyces pombe | - |
6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | Q9UUB1 | var. pombe | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Schizosaccharomyces pombe | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 17188, calculated from sequence | Schizosaccharomyces pombe |
pentamer | 5 * 17189, electrospray MS | Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Schizosaccharomyces pombe |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
riboflavin | pH 7.0, 37°C, wild-type enzyme | Schizosaccharomyces pombe |