Application | Comment | Organism |
---|---|---|
drug development | enzyme is an ideal target for the discovery of novel antibiotics against gram-negative pathogens as they have only one copy of murA gene in its genome | Escherichia coli |
drug development | enzyme is an ideal target for the discovery of novel antibiotics against gram-negative pathogens as they have only one copy of murA gene in its genome | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
cloning and expression of MurA using pMAL expression system in frame with the fusion maltose binding protein in Escherichia coli BL21 | Escherichia coli |
cloning and expression of MurA using pMAL expression system in frame with the fusion maltose binding protein in Escherichia coli BL21 | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
no effect of 10% DMSO on the activity of enzyme | Escherichia coli |
no effect of 10% DMSO on the activity of enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Escherichia coli | |
Cu2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Escherichia coli | |
Cu2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Pseudomonas aeruginosa | |
Fe2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Escherichia coli | |
Fe2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Pseudomonas aeruginosa | |
fosfomycin | - |
Escherichia coli | |
fosfomycin | - |
Pseudomonas aeruginosa | |
MnCl2 | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Escherichia coli | |
MnCl2 | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Pseudomonas aeruginosa | |
Zn2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Escherichia coli | |
Zn2+ | metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
phosphoenolpyruvate | 0.125 mM Tris-HCl, pH 7.5 | Pseudomonas aeruginosa | |
0.00084 | - |
phosphoenolpyruvate | 0.125 mM Tris-HCl, pH 7.5 | Escherichia coli | |
0.0178 | - |
UDP-N-acetyl-D-glucosamine | 0.125 mM Tris-HCl, pH 7.5 | Pseudomonas aeruginosa | |
0.036 | - |
UDP-N-acetyl-D-glucosamine | 0.125 mM Tris-HCl, pH 7.5 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
as fusion protein with maltose binding protein, determined by SDS-PAGE | Escherichia coli |
89000 | - |
as fusion protein with maltose binding protein, determined by SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Escherichia coli | - |
phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Pseudomonas aeruginosa | - |
phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MurA purification is done by using self packed amylose resin column | Escherichia coli |
MurA purification is done by using self packed amylose resin column | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Pseudomonas aeruginosa | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Escherichia coli |
MurA | - |
Pseudomonas aeruginosa |
UDP-N-acetylglucosamine enolpyruvyl transferase | - |
Escherichia coli |
UDP-N-acetylglucosamine enolpyruvyl transferase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
37 | - |
- |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
UDP-N-acetyl-D-glucosamine | the calculated value for UDP is 40 times higher than that of ADP | Escherichia coli | |
2.4 | - |
UDP-N-acetyl-D-glucosamine | the calculated value for UDP is 40times higher than that of ADP | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | enzyme shows activity at wide range of pH | Escherichia coli |
6 | 9 | enzyme shows activity at wide range of pH | Pseudomonas aeruginosa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00053 | - |
enzyme is pre-incubated with the inhibitor in the presence of 1 mM UDP-N-acetyl-D-glucosamine, reaction is started by addition of phosphoenolpyruvate | Pseudomonas aeruginosa | fosfomycin | |
0.00077 | - |
enzyme is pre-incubated with the inhibitor in the presence of 1 mM UDP-N-acetyl-D-glucosamine, reaction is started by addition of phosphoenolpyruvate | Escherichia coli | fosfomycin | |
0.0045 | - |
enzyme shows 5-7fold shift in IC50 for the inhibitor upon pre-incubation with the substrate UDP-N-acetyl-D-glucosamine | Pseudomonas aeruginosa | fosfomycin | |
0.0051 | - |
enzyme shows 5-7fold shift in IC50 for the inhibitor upon pre-incubation with the substrate UDP-N-acetyl-D-glucosamine | Escherichia coli | fosfomycin |
General Information | Comment | Organism |
---|---|---|
malfunction | gram-negative bacteria have only one copy of the murA gene, its deletion is lethal | Escherichia coli |
malfunction | gram-negative bacteria have only one copy of the murA gene, its deletion is lethal | Pseudomonas aeruginosa |
physiological function | enzyme catalyzes the first committed step of bacterial cell wall biosynthesis | Escherichia coli |
physiological function | enzyme catalyzes the first committed step of bacterial cell wall biosynthesis | Pseudomonas aeruginosa |