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Literature summary for 2.5.1.63 extracted from

  • Lowe, P.T.; Cobb, S.L.; OHagan, D.
    An enzymatic Finkelstein reaction fluorinase catalyses direct halogen exchange (2019), Org. Biomol. Chem., 17, 7493-7496 .
    View publication on PubMed
Search for more literature for 2.5.1.63

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya Q70GK9 bifunctional enzyme, catalyzes both the reaction of fluorinase EC 2.5.1.63 and chlorinase Ec 2.5.1.94
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 2'-deoxy-5'-fluoroadenosine + L-methionine
-
 r
5-deoxy-5'-bromoadenosine + fluoride
-
 Streptomyces cattleya 5-deoxy-5'-fluoroadenosine + bromide
-
 r
5-deoxy-5'-chloroadenosine + L-methionine
-
 Streptomyces cattleya S-adenosyl-L-methionine + chloride
-
 r
5-deoxy-5'-iodoadenosine + fluoride
-
 Streptomyces cattleya 5-deoxy-5'-fluoroadenosine + iodide
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.035
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya