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Literature summary for 2.5.1.56 extracted from

  • Yang, P.; Wang, J.; Pang, Q.; Zhang, F.; Wang, J.; Wang, Q.; Qi, Q.
    Pathway optimization and key enzyme evolution of N-acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor (2017), Metab. Eng., 43, 21-28 .
    View publication on PubMed

Application

Application Comment Organism
synthesis design of an in vivo NeuAc biosensor and application for genetic screening of a mutant library of NeuAc producers. A Ni2+-based selection system couples the cell growth with in vivo NeuAc concentration. The final strain produces up to 8.31 g/l NeuAc in minimal medium using glucose as sole carbon source Escherichia coli

Protein Variants

Protein Variants Comment Organism
A506G/G420A increase in specific activity Escherichia coli
C914T decrease in specific activity Escherichia coli
C914T/G1018A no significant change in specific activity Escherichia coli
G1018A no significant change in specific activity Escherichia coli
G283A no significant change in specific activity Escherichia coli
T74C increase in specific activity Escherichia coli
T74C/A506G increase in specific activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.93
-
mutant C914T, pH 7.0, 37°C Escherichia coli
26.5
-
mutant G1018A, pH 7.0, 37°C Escherichia coli
27.47
-
mutant C914T/G1018A, pH 7.0, 37°C Escherichia coli
28.11
-
wild-type, pH 7.0, 37°C Escherichia coli
28.96
-
mutant G283A, pH 7.0, 37°C Escherichia coli
30.76
-
mutant T74C, pH 7.0, 37°C Escherichia coli
32.48
-
mutant T74C/A506G, pH 7.0, 37°C Escherichia coli
35.71
-
mutant A506G/G420A, pH 7.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
Escherichia coli phosphate + N-acetylneuraminate
-
?
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
Escherichia coli K1 phosphate + N-acetylneuraminate
-
?