Application | Comment | Organism |
---|---|---|
synthesis | design of an in vivo NeuAc biosensor and application for genetic screening of a mutant library of NeuAc producers. A Ni2+-based selection system couples the cell growth with in vivo NeuAc concentration. The final strain produces up to 8.31 g/l NeuAc in minimal medium using glucose as sole carbon source | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A506G/G420A | increase in specific activity | Escherichia coli |
C914T | decrease in specific activity | Escherichia coli |
C914T/G1018A | no significant change in specific activity | Escherichia coli |
G1018A | no significant change in specific activity | Escherichia coli |
G283A | no significant change in specific activity | Escherichia coli |
T74C | increase in specific activity | Escherichia coli |
T74C/A506G | increase in specific activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli K1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.93 | - |
mutant C914T, pH 7.0, 37°C | Escherichia coli |
26.5 | - |
mutant G1018A, pH 7.0, 37°C | Escherichia coli |
27.47 | - |
mutant C914T/G1018A, pH 7.0, 37°C | Escherichia coli |
28.11 | - |
wild-type, pH 7.0, 37°C | Escherichia coli |
28.96 | - |
mutant G283A, pH 7.0, 37°C | Escherichia coli |
30.76 | - |
mutant T74C, pH 7.0, 37°C | Escherichia coli |
32.48 | - |
mutant T74C/A506G, pH 7.0, 37°C | Escherichia coli |
35.71 | - |
mutant A506G/G420A, pH 7.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | - |
Escherichia coli | phosphate + N-acetylneuraminate | - |
? | |
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | - |
Escherichia coli K1 | phosphate + N-acetylneuraminate | - |
? |