Application | Comment | Organism |
---|---|---|
synthesis | construction of a recombinant Escherichia coli simultaneously overexpressing GlcNAc 2-epimerase and N-acetylneuraminic acid synthase. The recombinant strain produces 25 g/l N-acetylneuraminic acid in 22 h without the addition of Corynebacterium ammoniagenes cells. For manufacturing on an industrial scale, it is preferable to use unconcentrated culture broth as the source of enzymes. The N-acetylneuraminic acid aldolase gene of the host strain is disrupted yielding a strain which cannot degrade N-acetylneuraminic acid. After a 22 h reaction with 120 g/l N-acetylglucosamine in a 5 l jar fermenter, the culture broth of contains 53 g/l N-acetylneuraminic acid | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
NeuB | - |
Escherichia coli |