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Literature summary for 2.5.1.56 extracted from

  • Vann, W.F.; Tavarez, J.J.; Crowley, J.; Vimr, E.; Silver, R.P.
    Purification and characterization of the Escherichia coli K1 neuB gene product N-acetylneuraminic acid synthetase (1997), Glycobiology, 7, 697-701.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal required, optimal activity with Mn2+ or Mg2+ Escherichia coli
Mn2+ divalent metal required Escherichia coli
Mn2+ optimal activity with Mn2+ or Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O Escherichia coli the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria ?
-
?
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O Escherichia coli K1 the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000416
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O specific for Escherichia coli N-acetylneuraminate + phosphate
-
?
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O specific for Escherichia coli K1 N-acetylneuraminate + phosphate
-
?
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria Escherichia coli ?
-
?
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria Escherichia coli K1 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Escherichia coli