Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is an anti-infective drug target | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
expression in Eschererichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor method | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2-[3'-[([1,1'-biphenyl]-4-sulfonyl)amino][1,1'-biphenyl]-3-yl]-1-hydroxyethane-1,1-diyl)bis(phosphonic acid) | - |
Staphylococcus aureus | |
1-(2,2-diphosphonoethyl)-3-(dodecyloxy)pyridin-1-ium | - |
Staphylococcus aureus | |
1-(2,2-diphosphonoethyl)-3-(heptyloxy)pyridin-1-ium | - |
Staphylococcus aureus | |
1-benzyl-3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-butyl-3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-butyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-decyl-3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-decyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-dodecyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-ethyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-heptyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
1-hexyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-(4-methoxyphenyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-dodecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-ethyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-hexyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-nonyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-octyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-pentadecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-tetradecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1-tridecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-methyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-nonyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-octyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-pentadecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-pentyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-propyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-tetradecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1-undecyl-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | - |
Staphylococcus aureus | |
3-(decyloxy)-1-(2,2-diphosphonoethyl)pyridin-1-ium | - |
Staphylococcus aureus | |
3-butoxy-1-(2,2-diphosphonoethyl)pyridin-1-ium | - |
Staphylococcus aureus | |
additional information | the most active inhibitors (Ki about 200 nM) are N-alkyl analogs of zoledronate containing C6 alkyl side-chains | Staphylococcus aureus | |
[2-[5-(decyloxy)pyridin-3-yl]ethane-1,1-diyl]bis(phosphonic acid) | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Staphylococcus aureus | |
0.041 | - |
isopentenyl diphosphate | pH and temperature not specified in the publication | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Staphylococcus aureus | - |
4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Staphylococcus aureus NCTC 8325 | - |
4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q2FYG6 | - |
- |
Staphylococcus aureus NCTC 8325 | Q2FYG6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | - |
Staphylococcus aureus | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | - |
Staphylococcus aureus NCTC 8325 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 22000 + 1 * 36000, SDS-PAGE, the enzyme consists of a catalytic subunit (HepPPS-2) having two DDXXD motifs and a regulatory subunit (HepPPS-1) that lacks these motifs | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
HepPPS | - |
Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Staphylococcus aureus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00083 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-hexyl-1H-imidazol-3-ium | |
0.001 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-(2,2-diphosphonoethyl)-3-(heptyloxy)pyridin-1-ium | |
0.0011 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-dodecyl-1H-imidazol-3-ium | |
0.0012 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-hexyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.0016 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-octyl-1H-imidazol-3-ium | |
0.002 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(decyloxy)-1-(2,2-diphosphonoethyl)pyridin-1-ium | |
0.002 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-butoxy-1-(2,2-diphosphonoethyl)pyridin-1-ium | |
0.0026 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-heptyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.0027 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-decyl-3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.0032 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-nonyl-1H-imidazol-3-ium | |
0.0047 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-octyl-1H-imidazol-3-ium | |
0.0052 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-pentyl-1H-imidazol-3-ium | |
0.0066 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-tridecyl-1H-imidazol-3-ium | |
0.0067 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-nonyl-1H-imidazol-3-ium | |
0.007 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-undecyl-1H-imidazol-3-ium | |
0.0091 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-decyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.017 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-tetradecyl-1H-imidazol-3-ium | |
0.042 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-butyl-3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.052 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-butyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.053 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-dodecyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.057 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | (2-[3'-[([1,1'-biphenyl]-4-sulfonyl)amino][1,1'-biphenyl]-3-yl]-1-hydroxyethane-1,1-diyl)bis(phosphonic acid) | |
0.072 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-pentadecyl-1H-imidazol-3-ium | |
0.077 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-tetradecyl-1H-imidazol-3-ium | |
0.082 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | [2-[5-(decyloxy)pyridin-3-yl]ethane-1,1-diyl]bis(phosphonic acid) | |
0.082 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.084 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-(4-methoxyphenyl)-1H-imidazol-3-ium | |
0.096 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1-ethyl-1H-imidazol-3-ium | |
0.099 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-propyl-1H-imidazol-3-ium | |
0.113 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-pentadecyl-1H-imidazol-3-ium | |
0.117 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-benzyl-3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.12 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-ethyl-3-(2-hydroxy-2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.136 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.139 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2-hydroxy-2,2-diphosphonoethyl)-1-methyl-1H-imidazol-3-ium | |
0.154 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 3-(2,2-diphosphonoethyl)-1H-imidazol-3-ium | |
0.166 | - |
pH and temperature not specified in the publication, mutant enzyme Y246D | Staphylococcus aureus | 1-(2,2-diphosphonoethyl)-3-(dodecyloxy)pyridin-1-ium |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the formation of menaquinone, a key electron transporter in many bacteria | Staphylococcus aureus |