Cloned (Comment) | Organism |
---|---|
gene cobAMm, expression in a Salmonella enterica strain lacking the housekeeping CobA enzyme restores coenzyme B12 synthesis, overexpression in Escherichia coli strain BL21(DE3) | Methanosarcina mazei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme expression restores coenzyme B12 synthesis in a Salmonella enterica strain lacking the housekeeping CobA enzyme | Methanosarcina mazei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 28% inhibition | Methanosarcina mazei | |
Mn2+ | 50% inhibition | Methanosarcina mazei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
cob(I)alamin | pH 8.0, 37°C | Methanosarcina mazei | |
0.003 | - |
ATP | pH 8.0, 37°C | Methanosarcina mazei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Methanosarcina mazei | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 2.5fold | Methanosarcina mazei | |
Co2+ | 1.75fold activation | Methanosarcina mazei | |
additional information | no activation by Na+ and Mg2+ | Methanosarcina mazei | |
Ni2+ | 1.5fold activation | Methanosarcina mazei | |
Zn2+ | 1.25fold activation | Methanosarcina mazei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ATP | Methanosarcina mazei | - |
adenosylcobalamin + triphosphate | - |
? | |
cob(I)inamide + ATP | Methanosarcina mazei | - |
adenosylcobinamide + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | - |
orf MM3138, housekeeping gene cobAMm | - |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange chromatography, dialysis, and hydrophobic interaction chromatography, recombinant enzyme from Escherichia coli strain BL21(DE3) | Methanosarcina mazei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
purified enzyme, pH 8.0 | Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ADP | - |
Methanosarcina mazei | adenosylcobalamin + diphosphate | - |
? | |
cob(I)alamin + ATP | - |
Methanosarcina mazei | adenosylcobalamin + triphosphate | - |
? | |
cob(I)alamin + ATP | cobalamin is a better substrate than cobinamide, the beta-phosphate of ATP is required for binding to the enzyme | Methanosarcina mazei | adenosylcobalamin + triphosphate | - |
? | |
cob(I)inamide + ATP | - |
Methanosarcina mazei | adenosylcobinamide + triphosphate | - |
? | |
cob(I)inamide + ATP | cobalamin is a better substrate than cobinamide, the beta-phosphate of ATP is required for binding to the enzyme | Methanosarcina mazei | adenosylcobinamide + triphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CobA-type ATP:Co(I)rrinoid adenosyltransferase | - |
Methanosarcina mazei |
CobAMm | - |
Methanosarcina mazei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina mazei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methanosarcina mazei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | 75% of maximal activity at pH 7.5, 94% at pH 8.5 | Methanosarcina mazei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Methanosarcina mazei | |
ATP | the beta-phosphate of ATP is required for binding to the enzyme | Methanosarcina mazei |