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Literature summary for 2.5.1.152 extracted from
- Biosynthesis of an opine metallophore by Pseudomonas aeruginosa (2017), Biochemistry, 56, 5967-5971 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cntL, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten steady-state kinetics, stopped flow spectrometry | Staphylococcus aureus | |
| 0.013 | - |
D-histidine | pH 8.0, 22°C, recombinant His-tagged enzyme | Staphylococcus aureus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + D-histidine | Staphylococcus aureus | - |
N-[(3S)-3-amino-3-carboxypropyl]-D-histidine + S-methyl-5'-thioadenosine | - |
? | |
| S-adenosyl-L-methionine + D-histidine | Staphylococcus aureus ATCC 700699 | - |
N-[(3S)-3-amino-3-carboxypropyl]-D-histidine + S-methyl-5'-thioadenosine | - |
? | |
| S-adenosyl-L-methionine + D-histidine | Staphylococcus aureus Mu50 | - |
N-[(3S)-3-amino-3-carboxypropyl]-D-histidine + S-methyl-5'-thioadenosine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | A0A0H3JXA8 | - |
- |
| Staphylococcus aureus ATCC 700699 | A0A0H3JXA8 | - |
- |
| Staphylococcus aureus Mu50 | A0A0H3JXA8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme SaNAS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration, to over 95% purity | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
purified recombinant His-tagged enzyme, with pyruvate and D-histidine, NADPH oxidation, pH 8,0, 22°C | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | SaNAS is specific for D-histidine. Coupled assay with enzyme staphylopine synthase from Staphylococcus aureus (SaODH, EC 1.5.1.52). Substrate specificities, overview | Staphylococcus aureus | ? | - |
- |
|
| additional information | SaNAS is specific for D-histidine. Coupled assay with enzyme staphylopine synthase from Staphylococcus aureus (SaODH, EC 1.5.1.52). Substrate specificities, overview | Staphylococcus aureus ATCC 700699 | ? | - |
- |
|
| additional information | SaNAS is specific for D-histidine. Coupled assay with enzyme staphylopine synthase from Staphylococcus aureus (SaODH, EC 1.5.1.52). Substrate specificities, overview | Staphylococcus aureus Mu50 | ? | - |
- |
|
| S-adenosyl-L-methionine + D-histidine | - |
Staphylococcus aureus | N-[(3S)-3-amino-3-carboxypropyl]-D-histidine + S-methyl-5'-thioadenosine | - |
? | |
| S-adenosyl-L-methionine + D-histidine | - |
Staphylococcus aureus ATCC 700699 | N-[(3S)-3-amino-3-carboxypropyl]-D-histidine + S-methyl-5'-thioadenosine | - |
? | |
| S-adenosyl-L-methionine + D-histidine | - |
Staphylococcus aureus Mu50 | N-[(3S)-3-amino-3-carboxypropyl]-D-histidine + S-methyl-5'-thioadenosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cntL | - |
Staphylococcus aureus |
| D-histidine 2-aminobutanoyltransferase | UniProt | Staphylococcus aureus |
| NAS | - |
Staphylococcus aureus |
| nicotianamine synthase-like enzyme | UniProt | Staphylococcus aureus |
| SaNAS | - |
Staphylococcus aureus |
| sav2468 | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.298 | - |
D-histidine | pH 8.0, 22°C, recombinant His-tagged enzyme | Staphylococcus aureus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Staphylococcus aureus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the NADPH is oxidized to NADP+ by the Staphylococcus aureus SaODH (EC 1.5.1.52) in the presence of nicotianamine synthase (PaNAS), S-adenosyl-L-methionine (SAM,) and the correct amino acid and 2-oxo acid substrates. The combination of D-histidine and pyruvate results in significant oxidation of NADPH by SaODH | Staphylococcus aureus |
| physiological function | opine dehydrogenases (ODHs) typically form a secondary amine by condensation of an amino acid with an alpha-keto acid. Staphylococcus aureus encodes the enzymes nicotianamine synthase (NAS) and opine dehydrogenase (ODH), biosynthesizing the nicotianamine-like opine metallophore staphylopaline. In Staphylococcus aureus, ODH uses the primary amine of the D-histidine-aminobutyrate product of NAS as a nucleophile in a condensation with pyruvate followed by hydride transfer from NADPH. SaNAS is specific for D-histidine | Staphylococcus aureus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22.92 | - |
D-histidine | pH 8.0, 22°C, recombinant His-tagged enzyme | Staphylococcus aureus | |
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