Literature summary for 2.5.1.132 extracted from

Lawrence, S.M.; Huddleston, K.A.; Pitts, L.R.; Nguyen, N.; Lee, Y.C.; Vann, W.F.; Coleman, T.A.; Betenbaugh, M.J.
Cloning and expression of the human N-acetylneuraminic acid phosphate synthase gene with 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid biosynthetic ability (2000), J. Biol. Chem., 275, 17869-17877.

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Cloned(Commentary)

Cloned (Comment)	Organism
the gene partially restores sialic acid synthase activity in a neuB-negative mutant of Escherichia coli and results in N-acetylneuraminic acid (Neu5Ac) and 2-dehydro-3-deoxy-D-glycero-D-galacto-nononic acid production in insect cells upon recombinant baculovirus infection	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9NR45	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + D-mannose 6-phosphate + H2O	the enzyme prefers N-acetyl-D-mannose 6-phosphate over D-mannose 6-phosphate in the production of phosphorylated forms of N-acetylneuraminic acid and 2-dehydro-3-deoxy-D-glycero-D-galacto-nononic acid	Homo sapiens	3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate	-	?
phosphoenolpyruvate + N-acetyl-D-mannosamine 6-phosphate + H2O	the enzyme prefers N-acetyl-D-mannose 6-phosphate over D-mannose 6-phosphate in the production of phosphorylated forms of N-acetylneuraminic acid and 2-dehydro-3-deoxy-D-glycero-D-galacto-nononic acid	Homo sapiens	? + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 40308, calculated from sequence	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)