Application | Comment | Organism |
---|---|---|
synthesis | the broad alkyl donor specificity of SirD opens the door for the interrogation of the alkyl donor specificity of other prenyltransferases for potential utility as biocatalysts for differential alkylation applications | Leptosphaeria maculans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leptosphaeria maculans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(2Z)-3-chlorobut-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.07 | - |
(2E)-but-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.12 | - |
(2E,4Z)-2-methylhexa-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.13 | - |
cyclohex-1-en-1-ylmethyl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.17 | - |
(2E)-2-methylpenta-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.2 | - |
(2E)-3-methylpent-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.21 | - |
2-cyclohexylideneethyl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.23 | - |
dimethylallyl diphosphate | with L-tyrosine as cosubstrate, at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.24 | - |
3-ethylpent-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.26 | - |
(2E)-penta-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.73 | - |
(2E)-5-methylhexa-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM used in assay conditions | Leptosphaeria maculans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tyrosine | Leptosphaeria maculans | - |
diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptosphaeria maculans | Q6Q874 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Leptosphaeria maculans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-methylpenta-2,4-dien-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-2-methylpenta-2,4-dien-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-3-(2-methoxyphenyl)prop-2-en-1-yl trihydrogen diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-3-(2-methoxyphenyl)prop-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-3-methylpent-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-3-methylpent-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-3-phenylbut-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-3-phenylbut-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-3-phenylprop-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-3-phenylprop-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-5-methylhexa-2,4-dien-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-5-methylhexa-2,4-dien-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-but-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-but-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E)-penta-2,4-dien-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E)-penta-2,4-dien-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E,4E)-2-methylhexa-2,4-dien-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E,4E)-2-methylhexa-2,4-dien-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2E,4E)-5-phenylpenta-2,4-dien-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2E,4E)-5-phenylpenta-2,4-dien-1-yl]-L-tyrosine + diphosphate | - |
? | |
(2Z)-3-chlorobut-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[(2Z)-3-chlorobut-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
2-cyclohexylideneethyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[2-cyclohexylideneethyl]-L-tyrosine + diphosphate | - |
? | |
2-methylprop-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[2-methylprop-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
2-oxopropyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[2-oxopropyl]-L-tyrosine + diphosphate | - |
? | |
3,3-diphenylprop-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[3,3-diphenylprop-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
3-ethylpent-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[3-ethylpent-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
cyanomethyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[cyanomethyl]-L-tyrosine + diphosphate | - |
? | |
cyclohex-1-en-1-ylmethyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[cyclohex-1-en-1-ylmethyl]-L-tyrosine + diphosphate | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Leptosphaeria maculans | diphosphate + 7-dimethylallyl-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
prop-2-en-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[prop-2-en-1-yl]-L-tyrosine + diphosphate | - |
? | |
prop-2-yn-1-yl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | O-[prop-2-yn-1-yl]-L-tyrosine + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SirD | - |
Leptosphaeria maculans |
tyrosine O-prenyltransferase | - |
Leptosphaeria maculans |
tyrosine-O-prenyltransferase | - |
Leptosphaeria maculans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
(2Z)-3-chlorobut-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.00083 | - |
(2E)-penta-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.001 | - |
(2E)-but-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.00167 | - |
(2E)-5-methylhexa-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.00367 | - |
(2E)-2-methylpenta-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.0055 | - |
cyclohex-1-en-1-ylmethyl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.0123 | - |
(2E,4Z)-2-methylhexa-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.055 | - |
3-ethylpent-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.0583 | - |
2-cyclohexylideneethyl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.3 | - |
(2E)-3-methylpent-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.683 | - |
dimethylallyl diphosphate | with L-tyrosine as cosubstrate, at pH 7.5 and 35°C | Leptosphaeria maculans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
(2E)-5-methylhexa-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.0033 | - |
(2E)-penta-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.015 | - |
(2E)-but-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.0167 | - |
(2Z)-3-chlorobut-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.0217 | - |
(2E)-2-methylpenta-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.042 | - |
cyclohex-1-en-1-ylmethyl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.1017 | - |
(2E,4Z)-2-methylhexa-2,4-dien-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.23 | - |
3-ethylpent-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
0.267 | - |
2-cyclohexylideneethyl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
1.5 | - |
(2E)-3-methylpent-2-en-1-yl diphosphate | at pH 7.5 and 35°C | Leptosphaeria maculans | |
2.97 | - |
dimethylallyl diphosphate | with L-tyrosine as cosubstrate, at pH 7.5 and 35°C | Leptosphaeria maculans |