Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 2.5 mM, increases activity 2fold | Mycobacterium tuberculosis | |
Triton X-100 | 0.2%, 1.5fold increase in activity | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression in Mycobacterium smegmatis using a new mycobacterial expression vector that encodes an in-frame N-terminal affinity tag fusion with the protein of interest | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | above 0.2% | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0098 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.043 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | absolutely dependent on presence of divalent cation. Supports activity to a lower level than Mg2+ | Mycobacterium tuberculosis | |
Mg2+ | absolutely dependent on presence of divalent cation. Optimal activity at 4-10 mM | Mycobacterium tuberculosis | |
Mn2+ | absolutely dependent on presence of divalent cation. Supports activity to a lower level than Mg2+ | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
fusion protein | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + isopentenyl diphosphate | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis | diphosphate + trans,trans-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis H37Rv | diphosphate + trans,trans-farnesyl diphosphate | - |
? | |
additional information | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
omega,E,E-farnesyl diphosphate synthase | - |
Mycobacterium tuberculosis |
omega,E,E-FPP | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.49 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | active over the range pH 7-10 | Mycobacterium tuberculosis |