General Stability | Organism |
---|---|
strongly stabilized by addition of phospholipids upon detergent solubilization, phosphatidylcholine is twice as effective as phosphatidylethanolamine | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sus scrofa | 16020 | - |
membrane | bound to | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Gallus gallus | |
Mg2+ | absolute requirement for divalent cations that can adopt an octahedral coordination geometry with preference for Mn2+ over Mg2+ | Saccharomyces cerevisiae | |
Mn2+ | - |
Gallus gallus | |
Mn2+ | absolute requirement for divalent cations that can adopt an octahedral coordination geometry with preference for Mn2+ over Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6000 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
9500 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
16000 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
30000 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
32000 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
34000 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
47000 | - |
x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | Gallus gallus | - |
? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | Canis lupus familiaris | - |
? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | Saccharomyces cerevisiae | N-glycosylation of eukaryotic secretory and membrane-bound proteins is an essential and highly conserved protein modification. The key step in this pathway is the en bloc transfer of the high mannose core oligosaccharide Gly3Man9GlcNAc2 from the lipid carries dolichyl phosphate to selected Asn-X-Ser/Thr sequences of nascent polypeptide chains during their translocation across the endoplasmic reticulum membrane | ? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | Saccharomyces cerevisiae | dolichyl-diphosphochitobiose-Man9Glc3 is the preferred glycosyl donor both in vivo and in vitro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Gallus gallus | - |
- |
- |
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | P48439 | - |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Sus scrofa | - |
oviduct | - |
Gallus gallus | - |
pancreas | - |
Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl diphosphooligosaccharide + protein L-asparagine | - |
Gallus gallus | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
dolichyl diphosphooligosaccharide + protein L-asparagine | - |
Homo sapiens | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
dolichyl diphosphooligosaccharide + protein L-asparagine | - |
Sus scrofa | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
dolichyl diphosphooligosaccharide + protein L-asparagine | - |
Canis lupus familiaris | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
dolichyl diphosphooligosaccharide + protein L-asparagine | dolichyl-diphosphochitobiose-Man9Glc3 is the preferred glycosyl donor both in vivo and in vitro. The minimal glycosyl donor is dolichyl-diphosphochitobiose | Saccharomyces cerevisiae | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | - |
Gallus gallus | ? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | - |
Canis lupus familiaris | ? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | N-glycosylation of eukaryotic secretory and membrane-bound proteins is an essential and highly conserved protein modification. The key step in this pathway is the en bloc transfer of the high mannose core oligosaccharide Gly3Man9GlcNAc2 from the lipid carries dolichyl phosphate to selected Asn-X-Ser/Thr sequences of nascent polypeptide chains during their translocation across the endoplasmic reticulum membrane | Saccharomyces cerevisiae | ? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | dolichyl-diphosphochitobiose-Man9Glc3 is the preferred glycosyl donor both in vivo and in vitro | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Gallus gallus |
? | - |
Sus scrofa |
? | - |
Canis lupus familiaris |
? | x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Saccharomyces cerevisiae |