Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.18 mM, 392% activation of purified sialyltransferase-1, 1120% activation of sialytransferase-1 activity in microsomes | Rattus norvegicus |
General Stability | Organism |
---|---|
PMSF, leupeptin or pepstatin A stabilize during purification | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4-Amidinophenyl)-methanesulfonyl fluoride | serine protease, 0.02 mM, 48% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
alpha2-Macroglobulin | 1 unit, 67% inhibition of purified sialyltransferase-1, no inhibition of sialyltransferase-1 activity in microsomes | Rattus norvegicus | |
Aprotinin | serine protease inhibitor, 0.0003 mM, 74% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
dithiothreitol | 0.1 mM, 89% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
E-64 | thiol protease inhibitor, 0.0028 mM, 71% inhibition of purified sialyltransferase-1, activation of sialytransferase-1 activity in microsomes | Rattus norvegicus | |
EDTA | 0.1 mM, 71% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
Ep-475 | thiol protease inhibitor, 0.0028 mM, 75% inhibition of purified sialyltransferase-1; thiol protease inhibitor, 0.0028 mM, 84% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone | chymotrypsin and thiol protease inhibitor, 0.284 mM, 67% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
L-1-Chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl | trypsin and thiol protease inhibitor, 0.135 mM, 73% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
leupeptin | serine and thiol protease inhibitor, 0.001 mM, 72% inhibition of purified sialyltransferase-1, no inhibition of sialyltransferase-1 activity in microsomes | Rattus norvegicus | |
additional information | inhibition in vivo i.e. stable microsomes not as dramatic as in vitro | Rattus norvegicus | |
N3- | 1 mM, 87% inhibition of purified sialyltransferase-1 | Rattus norvegicus | |
pepstatin A | 0.001 mM, 72% inhibition of purified sialyltransferase-1, activation of sialyltransferase-1 activity in microsomes | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
Rattus norvegicus | CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | i.e. ganglioside GM3 | ? |