Literature summary for 2.4.3.9 extracted from

Fishman, P.H.; Bradley, R.M.; Henneberry, R.C.
Butyrate-induced glycolipid biosynthesis in HeLa cells: properties of the induced sialyltransferase (1976), Arch. Biochem. Biophys., 172, 618-626.

Search for more literature for 2.4.3.9

Activating Compound

Activating Compound	Comment	Organism	Structure
cardiolipin	activation	Homo sapiens
Cutscum	activation, most potent activator	Homo sapiens
Cutscum	can be replaced by Triton CF-54, Triton X-100 or Triton CF-54/Tween 80, ratio 2:1, activation efficiency in descending order	Homo sapiens
Nonidet P-40	slight activation	Homo sapiens
Triton CF-54	-	Homo sapiens
Triton CF-54/Tween 80	ratio 2:1, activation, about 50% as efficient as Cutscum	Homo sapiens
Triton X-100	activation	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibited by EDTA	Homo sapiens
Tris buffer	weak	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	lactosylceramide	-	Homo sapiens
1.5	-	CMP-N-acetylneuraminate	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no metal ion requirement	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	strain R, elevated enzyme level if cultured in butyrate containing media	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0000045	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide	-	Homo sapiens	CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide	i.e. ganglioside GM3	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	5 min, inactivation	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Homo sapiens

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Release 2023.1 (January 2023)