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Literature summary for 2.4.3.8 extracted from
- Purification to homogeneity of GD3 synthase and partial purification of GM3 synthase from rat brain (1990), Biochem. Biophys. Res. Commun., 166, 387-393.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Myrj 59 | activation, most potent activator, can be replaced by the following detergents, descending efficiency: sodium deoxycholate, Triton CF-54, Tween 20, Tween 80/Triton CF-54 ratio 1:*2, Triton X-100 or Tween 80 | Rattus norvegicus |  |
| Triton CF-54 | - |
Rattus norvegicus | |
| Triton X-100 | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-gel electrophoresis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
12-14 days old | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized with Triton X-100, CDP-Sepharose affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| pancreas | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0189 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | i.e. disialosyllactosylceramide or ganglioside GD3 | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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