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Literature summary for 2.4.3.6 extracted from
- Purification, cloning, and expression of an alpha/beta-galactoside alpha-2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum (2007), J. Biol. Chem., 282, 29794-29802 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of full-length and truncated protein in Escherichia coli | Photobacterium phosphoreum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
CMP-N-acetyl-beta-neuraminate | pH 6.0, 25°C | Photobacterium phosphoreum |  |
| 1.7 | - |
beta-D-Gal-(1->4)-beta-D-Glc | pH 6.0, 25°C | Photobacterium phosphoreum | |
| 2.5 | - |
beta-D-Gal-(1->4)-beta-D-GlcNAc | pH 6.0, 25°C | Photobacterium phosphoreum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | native and recombinant enzymes are twice as active in the presence of NaCl at 500, 750, and 1000 mM as in the absence of NaCl | Photobacterium phosphoreum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Photobacterium phosphoreum |
| 45026 | - |
MALDI-TOF MS analysis | Photobacterium phosphoreum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photobacterium phosphoreum | A5LHX0 | - |
- |
| Photobacterium phosphoreum JT-ISH-467 | A5LHX0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Photobacterium phosphoreum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-Glc | - |
Photobacterium phosphoreum | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-Glc | - |
Photobacterium phosphoreum JT-ISH-467 | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc | 103% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc | 103% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum JT-ISH-467 | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-mannopyranoside | 43% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + alpha-Neu5Ac-(2->3)-beta-D-Man | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-mannopyranoside | 43% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum JT-ISH-467 | CMP + alpha-Neu5Ac-(2->3)-beta-D-Man | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylglucosamine | 40% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + alpha-Neu5Ac-(2->3)-beta-D-GlcNAc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylglucosamine | 40% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum JT-ISH-467 | CMP + alpha-Neu5Ac-(2->3)-beta-D-GlcNAc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->4)-beta-D-GlcNAc | 129% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->4)-beta-D-GlcNAc | 129% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum JT-ISH-467 | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + methyl beta-D-galactopyranoside | 251% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal | - |
? | |
| CMP-N-acetyl-beta-neuraminate + methyl beta-D-glucopyranoside | 33% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Glc | - |
? | |
| CMP-N-acetyl-beta-neuraminate + methyl beta-D-mannopyranoside | 63% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme | Photobacterium phosphoreum | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Man | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Photobacterium phosphoreum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 25 | native enzyme and the full-length recombinant enzyme | Photobacterium phosphoreum |
| 25 | 30 | truncated recombinant enzyme | Photobacterium phosphoreum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | native enzyme and the full-length recombinant enzyme | Photobacterium phosphoreum |
| 5.5 | 6 | truncated recombinant enzyme | Photobacterium phosphoreum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | active between pH 5.0 and pH 11.0, native enzyme and recombinant enzyme | Photobacterium phosphoreum |
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