Literature summary for 2.4.2.28 extracted from

Torini, J.R.; Brandao-Neto, J.; DeMarco, R.; Pereira, H.D.
Crystal structure of Schistosoma mansoni adenosine phosphorylase/5'-methylthioadenosine phosphorylase and its importance on adenosine salvage pathway (2016), PLoS Negl. Trop. Dis., 10, e0005178 .

Search for more literature for 2.4.2.28

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with adenine, 5'-deoxy-5'-methylthioadenosine, tubercidin or sulfate, hanging drop vapor diffusion method, using 100 mM Bis-Tris or MES buffer, pH 6.1-6.7 and 14-18% (w/v) PEG 3350	Schistosoma mansoni

Protein Variants

Protein Variants	Comment	Organism
N87T	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Schistosoma mansoni
Q289L	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Schistosoma mansoni
S12T	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Schistosoma mansoni
S12T/N87T	the mutant shows increased catalytic efficiencies with 5'-deoxy-5'-methylthioadenosine and 2'-deoxyadenosine and decreased catalytic efficiency with adenosine compared to the wild type enzyme	Schistosoma mansoni
S12T/N87T/Q289L	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Schistosoma mansoni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00071	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00099	-	2'-deoxyadenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00122	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00181	-	adenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
0.0021	-	adenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
0.002124	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00249	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
0.00302	-	2'-deoxyadenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
0.00312	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
0.00314	-	adenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
0.0033	-	adenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00363	-	5'-deoxy-5'-methylthioadenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
0.00368	-	adenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00389	-	2'-deoxyadenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
0.00397	-	2'-deoxyadenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
0.00652	-	2'-deoxyadenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
0.00735	-	adenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
0.01033	-	2'-deoxyadenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5'-deoxy-5'-methylthioadenosine + phosphate	Schistosoma mansoni	-	adenine + 5'-deoxy-5'-methyl-thio-D-ribose-1-phosphate	-	?
adenosine + phosphate	Schistosoma mansoni	the enzyme can use adenosine with the same efficiency as 5'-deoxy-5'-methylthioadenosine	adenine + D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Schistosoma mansoni	I0B503	-	-

Purification (Commentary)

Purification (Comment)	Organism
Co-NTA agarose column chromatography	Schistosoma mansoni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2'-deoxyadenosine + phosphate	the enzyme can use adenosine with the same efficiency as 5'-deoxy-5'-methylthioadenosine	Schistosoma mansoni	adenine + 2'-deoxy-D-ribose 1-phosphate	-	?
5'-deoxy-5'-methylthioadenosine + phosphate	-	Schistosoma mansoni	adenine + 5'-deoxy-5'-methyl-thio-D-ribose-1-phosphate	-	?
adenosine + phosphate	the enzyme can use adenosine with the same efficiency as 5'-deoxy-5'-methylthioadenosine	Schistosoma mansoni	adenine + D-ribose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 33900, SDS-PAGE	Schistosoma mansoni
?	x * 32914, calculated from amino acid sequence	Schistosoma mansoni

Synonyms

Synonyms	Comment	Organism
5'-deoxy-5'-methylthioadenosine phosphorylase	-	Schistosoma mansoni
5'-methylthioadenosine phosphorylase	-	Schistosoma mansoni
MTAP	-	Schistosoma mansoni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
48.44	-	2'-deoxyadenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
62.63	-	adenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
80.03	-	2'-deoxyadenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
84.62	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
95.79	-	adenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
98.42	-	adenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
145.1	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
224.3	-	2'-deoxyadenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
241.6	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
253.9	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
280.3	-	adenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
299.2	-	2'-deoxyadenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
308.9	-	adenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
334.1	-	adenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
372.8	-	2'-deoxyadenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
416.2	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
441.2	-	2'-deoxyadenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
443.8	-	5'-deoxy-5'-methylthioadenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni

General Information

General Information	Comment	Organism
metabolism	the enzyme is part of the purine pathway salvage	Schistosoma mansoni

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
18.97	-	adenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
26.5	-	2'-deoxyadenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
38.13	-	adenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
42.71	-	2'-deoxyadenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
45.88	-	2'-deoxyadenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
46.86	-	adenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
48.92	-	2'-deoxyadenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
54.58	-	adenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
56.49	-	2'-deoxyadenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
81.37	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
83.94	-	adenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
95.83	-	2'-deoxyadenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni
97.02	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme Q289L, at pH 7.4 and 25°C	Schistosoma mansoni
106.4	-	adenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
118.93	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T, at pH 7.4 and 25°C	Schistosoma mansoni
119.18	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme N87T, at pH 7.4 and 25°C	Schistosoma mansoni
122.25	-	5'-deoxy-5'-methylthioadenosine	wild type enzyme, at pH 7.4 and 25°C	Schistosoma mansoni
196.32	-	5'-deoxy-5'-methylthioadenosine	mutant enzyme S12T/N87T, at pH 7.4 and 25°C	Schistosoma mansoni

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Release 2023.1 (January 2023)