General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Columba livia |
prolonged dialysis against distilled water leads to precipitation | Columba livia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Columba livia | |
AMP | - |
Columba livia | |
AMP | - |
Rattus norvegicus | |
ATP | - |
Columba livia | |
GDP | - |
Columba livia | |
GMP | - |
Columba livia | |
GMP | - |
Rattus norvegicus | |
IMP | - |
Columba livia | |
additional information | not inhibited by ribose 5-phosphate, purine ribonucleosides or bases, 2'- or 3'-phosphate or deoxyribose phosphate analogues or by pyrimidine ribonucleotides | Columba livia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.086 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Rattus norvegicus | |
0.1 | - |
L-glutamine | - |
Columba livia | |
0.24 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Columba livia | |
0.53 | - |
L-glutamine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
soluble | - |
Columba livia | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Columba livia | - |
pigeon | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, heat treatment, calcium phosphate gel, DEAE-cellulose | Columba livia |
partial | Rattus norvegicus |
partial | Columba livia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Columba livia | - |
Storage Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during storage | Columba livia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Rattus norvegicus | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Columba livia | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
6 min, about 25% loss of activity | Columba livia |
60 | - |
after 3-6 min loss of ATP-sensitivity | Columba livia |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | 1.1 | ATP | - |
Columba livia | |
0.038 | 0.64 | ADP | - |
Columba livia | |
0.086 | 0.35 | GMP | - |
Columba livia | |
0.092 | 2.5 | AMP | - |
Columba livia | |
0.18 | 3.5 | IMP | - |
Columba livia | |
0.38 | 5.4 | GDP | - |
Columba livia |