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Literature summary for 2.4.2.1 extracted from

  • Prokopowicz, M.; Ciesla, J.; Kierdaszuk, B.
    A synergistic effect of phosphate, pH and Phe159 substitution on the formycin A association to the E. coli purine nucleoside phosphorylase (2018), Biochimie, 148, 80-86 .
    View publication on PubMed
Search for more literature for 2.4.2.1

Protein Variants

Protein Variants Comment Organism
F159A site-directed mutagenesis, the PNPF159A-FA complexes show a weak association of formycin A to the mutant's active center Escherichia coli
F159Y site-directed mutagenesis, a prominent quenching of the PNPF159Y emission indicates a complex formation, with the strongest association in the phosphate buffer, pH 7.0, relative to the wild-type enzyme. On the other hand, results testify to a deterioration of the interactions in the wild-type PNP/PNPF159Y mutant and formycin A complexes in the presence of the phosphate, pH 8.3 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Formycin A a potent inhibitor of hexameric PNPs. Inhibitor-enzyme interaction and kinetic analysis with wild-type and mutant PNPs, detailed overview. With the wild-type enzyme, in the P8.3 at 10°C a model of binding one molecule per enzyme hexamer gives the best fit and at 25°C all types of fits are comparable. The strongest association is observed in the phosphate buffer pH 7.0. On the other hand, results show that the presence of phosphate at pH 8.3 is responsible for a strong binding impairment (Kd increase). The behaviour of the Kapp calculated for the PNPY-FA complexes corresponds to the Kapp of the PNPWT-FA. Their values suggest stronger than in the PNPWT-FA complexes association in the P7 and P8.3 at 10°C and in H8.3, P7 and P8.3 at 25°C Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics and kinetic analysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ABP8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
homohexamer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
PNP
-
 Escherichia coli
purine nucleoside phosphorylase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10 25 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.3 assay at Escherichia coli