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Literature summary for 2.4.1.B34 extracted from
- The Gram-negative bacterium Azotobacter chroococcum NCIMB 8003 employs a new glycoside hydrolase family 70 4,6-alpha-glucanotransferase enzyme (GtfD) to synthesize a reuteran like polymer from maltodextrins and starch (2016), Biochim. Biophys. Acta, 1860, 1224-1236.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 86400 | - |
x * 90000, SDS-PAGE, x * 86400, calculated | Azotobacter chroococcum |
| 90000 | - |
x * 90000, SDS-PAGE, x * 86400, calculated | Azotobacter chroococcum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter chroococcum | A0A0C4WTK3 | - |
- |
| Azotobacter chroococcum NCIMB 8003 | A0A0C4WTK3 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
pH 6.5, 50°C | Azotobacter chroococcum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose V | - |
Azotobacter chroococcum | ? | GtfB produces a high molecular mass polymer with a molecular mass about 80 times greater than that of the starting amylose V | ? |  |
| amylose V + maltose | - |
Azotobacter chroococcum | maltotriose + maltotetraose + ? | - |
? | |
| Maltoheptaose | - |
Azotobacter chroococcum | ? | - |
? | |
| Maltoheptaose | - |
Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
| Maltohexaose | - |
Azotobacter chroococcum | ? | - |
? | |
| Maltohexaose | - |
Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
| maltopentaose | - |
Azotobacter chroococcum | ? | - |
? | |
| maltose | - |
Azotobacter chroococcum | ? | - |
? | |
| Maltotetraose | - |
Azotobacter chroococcum | ? | - |
? | |
| Maltotetraose | - |
Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
| maltotriose | - |
Azotobacter chroococcum | ? | - |
? | |
| additional information | GtfD shows clear hydrolase/transglycosylase activity with malto-oligosaccharides with degree of polymerzation of 3 to 7 and forms a range of shorter and longer oligosaccharides. The enzyme is unable to synthesize consecutive alpha1->6 glucosidic bonds. Instead, it forms a high molecular mass and branched alpha-glucan with alternating alpha1->4 and alpha1->6 linkages from amylose/starch | Azotobacter chroococcum | ? | - |
? | |
| additional information | inactive on sucrose, panose, nigerose, beta-cyclodextrins, and isomalto-oligosaccharides with degree of polymerzation of 2, 3, and 5 | Azotobacter chroococcum | ? | - |
? | |
| additional information | GtfD shows clear hydrolase/transglycosylase activity with malto-oligosaccharides with degree of polymerzation of 3 to 7 and forms a range of shorter and longer oligosaccharides. The enzyme is unable to synthesize consecutive alpha1->6 glucosidic bonds. Instead, it forms a high molecular mass and branched alpha-glucan with alternating alpha1->4 and alpha1->6 linkages from amylose/starch | Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
| additional information | inactive on sucrose, panose, nigerose, beta-cyclodextrins, and isomalto-oligosaccharides with degree of polymerzation of 2, 3, and 5 | Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
| potato starch | - |
Azotobacter chroococcum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE, x * 86400, calculated | Azotobacter chroococcum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Achr_35950 | - |
Azotobacter chroococcum |
| GtfD | - |
Azotobacter chroococcum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Azotobacter chroococcum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for at least 10 min | Azotobacter chroococcum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Azotobacter chroococcum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | more than 70% of maximum activity | Azotobacter chroococcum |
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