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Literature summary for 2.4.1.B34 extracted from

  • Gangoiti, J.; Pijning, T.; Dijkhuizen, L.
    The Exiguobacterium sibiricum 255-15 GtfC enzyme represents a novel glycoside hydrolase 70 subfamily of 4,6-alpha-glucanotransferase enzymes (2016), Appl. Environ. Microbiol., 82, 756-766.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B34

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 1 mM, slight activation Exiguobacterium sibiricum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Exiguobacterium sibiricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99000
-
 x * 99000, calculated Exiguobacterium sibiricum

Organism

Organism UniProt Comment Textmining
Exiguobacterium sibiricum B1YMN6 enzyme GtfC differs in the domain order from dextransucrase and GtfB enzymes, and it completely lacks domain V
-
Exiguobacterium sibiricum DSM 17290 B1YMN6 enzyme GtfC differs in the domain order from dextransucrase and GtfB enzymes, and it completely lacks domain V
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
 40°C, pH 6.0 Exiguobacterium sibiricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose V
-
 Exiguobacterium sibiricum ?
-
 ?
amylose V
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
maltoheptaose + H2O
-
 Exiguobacterium sibiricum D-glucose + maltotriose + maltohexaose first clear products of the reaction ?
Maltohexaose
-
 Exiguobacterium sibiricum ?
-
 ?
Maltohexaose
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
maltopentaose
-
 Exiguobacterium sibiricum ?
-
 ?
maltopentaose
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
Maltotetraose
-
 Exiguobacterium sibiricum ?
-
 ?
Maltotetraose
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
additional information GtfC acts on maltooligosaccharides and amylose-V yielding linear gluco-oligomers also containing, besides alpha1->4, alpha1->6 glycosidic linkages. In the product mixture, free glucose units, 4-substituted reducing-end glucose residues and trace amounts of 6-substituted reducing-end glucose residues are present. The higher the degree of polymerization of the substrate, the higher the percentages of alpha1->6 glycosidic linkages introduced into the product. No substrates: maltose, maltotriose, sucrose, nigerose, isomaltooliogosaccharides, panose, reuteran Exiguobacterium sibiricum ?
-
 ?
additional information GtfC acts on maltooligosaccharides and amylose-V yielding linear gluco-oligomers also containing, besides alpha1->4, alpha1->6 glycosidic linkages. In the product mixture, free glucose units, 4-substituted reducing-end glucose residues and trace amounts of 6-substituted reducing-end glucose residues are present. The higher the degree of polymerization of the substrate, the higher the percentages of alpha1->6 glycosidic linkages introduced into the product. No substrates: maltose, maltotriose, sucrose, nigerose, isomaltooliogosaccharides, panose, reuteran Exiguobacterium sibiricum DSM 17290 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 99000, calculated Exiguobacterium sibiricum

Synonyms

Synonyms Comment Organism
Exig_2648
-
 Exiguobacterium sibiricum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Exiguobacterium sibiricum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 30% of maximum activity Exiguobacterium sibiricum
55
-
 drastic decrease above Exiguobacterium sibiricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Exiguobacterium sibiricum

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 more than 50% of maximum activity Exiguobacterium sibiricum