Any feedback?
Literature summary for 2.4.1.9 extracted from
- Recent advances in levansucrase and inulosucrase evolution, characteristics, and application (2019), Crit. Rev. Food Sci. Nutr., 59, 3630-3647 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Limosilactobacillus reuteri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D520A | the mutation causes a strong reduction of activity compared to the wild type enzyme | Limosilactobacillus reuteri |
| D520N | the mutation causes a strong reduction of activity compared to the wild type enzyme | Limosilactobacillus reuteri |
| R423H | the mutant shows 0.3% of wild type activity | Limosilactobacillus reuteri |
| R423K | the mutant shows 1.4% of wild type activity | Limosilactobacillus reuteri |
| W271N | the mutant shows 5.3% of wild type activity. The mutant synthesizes very similar amounts of fructooligosaccarides up to DP 6, but clearly less of DP 7-9 | Limosilactobacillus reuteri |
| W340N | the mutant shows 0.5% of wild type activity and only synthesizes small amounts of kestose and nystose | Limosilactobacillus reuteri |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Limosilactobacillus reuteri |  |
| Ca2+ | activates | Leuconostoc citreum | |
| Mn2+ | activates | Lactobacillus gasseri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + [(2->1)-beta-D-fructosyl]n | Limosilactobacillus reuteri | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Leuconostoc citreum | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Lactobacillus johnsonii | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Lactobacillus gasseri | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Lactobacillus johnsonii NCC 533 | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Leuconostoc citreum CW28 | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Limosilactobacillus reuteri 121 | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Limosilactobacillus reuteri TMW1.106 | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Lactobacillus gasseri DSM 20604 | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gasseri | D3WYV9 | - |
- |
| Lactobacillus gasseri DSM 20604 | D3WYV9 | - |
- |
| Lactobacillus johnsonii | Q74K42 | - |
- |
| Lactobacillus johnsonii NCC 533 | Q74K42 | - |
- |
| Leuconostoc citreum | - |
- |
- |
| Leuconostoc citreum CW28 | - |
- |
- |
| Limosilactobacillus reuteri | - |
- |
- |
| Limosilactobacillus reuteri | Q0VJC5 | - |
- |
| Limosilactobacillus reuteri 121 | - |
- |
- |
| Limosilactobacillus reuteri TMW1.106 | Q0VJC5 | - |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10% (v/v) glycerol, almost no loss of activity | Limosilactobacillus reuteri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + sucrose | - |
Limosilactobacillus reuteri | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Leuconostoc citreum | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Lactobacillus johnsonii | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Lactobacillus gasseri | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Lactobacillus johnsonii NCC 533 | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Leuconostoc citreum CW28 | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Limosilactobacillus reuteri 121 | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Limosilactobacillus reuteri TMW1.106 | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + sucrose | - |
Lactobacillus gasseri DSM 20604 | alpha-D-glucose + inulin-type oligosaccharide + inulin | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Limosilactobacillus reuteri | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Leuconostoc citreum | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus johnsonii | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus gasseri | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus johnsonii NCC 533 | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Leuconostoc citreum CW28 | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Limosilactobacillus reuteri 121 | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Limosilactobacillus reuteri TMW1.106 | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus gasseri DSM 20604 | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Leuconostoc citreum |
| ? | x * 63000, SDS-PAGE | Lactobacillus gasseri |
| ? | x * 87200, SDS-PAGE | Lactobacillus johnsonii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase | - |
Limosilactobacillus reuteri |
| sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase | - |
Leuconostoc citreum |
| sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase | - |
Lactobacillus johnsonii |
| sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase | - |
Lactobacillus gasseri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Leuconostoc citreum |
| 35 | - |
- |
Lactobacillus gasseri |
| 50 | - |
- |
Limosilactobacillus reuteri |
| 55 | - |
- |
Lactobacillus johnsonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Lactobacillus johnsonii |
| 5 | 5.5 | - |
Limosilactobacillus reuteri |
| 5.5 | - |
- |
Lactobacillus gasseri |
| 6.5 | - |
- |
Leuconostoc citreum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
