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Literature summary for 2.4.1.66 extracted from

  • Smith, D.F.; Wu, C.; Jamieson, G.A.
    Characterization of human platelet UDPglucose-collagen glucosyltransferase using a new rapid assay (1977), Biochim. Biophys. Acta, 483, 263-278.
    View publication on PubMed

General Stability

General Stability Organism
chromatography on Biogel A-1.5M decreases stability Homo sapiens
extremely stable to freezing, -40°C, and thawing cycles, soluble enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ soluble enzyme, above 50 mM Homo sapiens
additional information membrane-bound enzyme: not inhibited by 100 mM D-glucosamine, 10 mM N-ethylmaleimide, 50 mM glucosylgalactosylhydroxylysine, soluble enzyme: not inhibited by 75 mM glucosylgalactosylhydroxylysine Homo sapiens
p-chloromercuribenzoate membrane-bound enzyme, 6 and 10 mM: 54% inhibition Homo sapiens
sucrose membrane-bound enzyme, 0.42 M: 88% inhibition, also inhibits soluble enzyme Homo sapiens
UDP competitive to UDP-glucose; membrane-bound enzyme, 6 mM: complete inhibition; non-competitive to galactosylhydroxylysine; product inhibition Homo sapiens
UTP membrane-bound enzyme, 6 mM: complete inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Homo sapiens
additional information
-
additional information Km: 1.5 mg/ml, alpha-chain of chicken skin collagen Homo sapiens
0.002
-
D-galactosylhydroxylysine bound in purified alpha1-chain of chicken skin collagen, value based on actual amount of galactosylhydroxylysine-residues of alpha1-chain Homo sapiens
0.005
-
UDP-glucose membrane-bound enzyme Homo sapiens
0.029
-
TDP-glucose soluble enzyme Homo sapiens
3.8
-
D-galactosylhydroxylysine membrane-bound enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm 5-10% of the total activity is surface-bound, the rest is of cytoplasmic origin Homo sapiens 5737
-
membrane membrane-bound Homo sapiens 16020
-
plasma membrane 5-10% of the total activity is surface-bound, the rest is of cytoplasmic origin Homo sapiens 5886
-
soluble
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 64% as effective as Mn2+ Homo sapiens
Mg2+ 14% as effective as Mn2+ Homo sapiens
Mn2+ requirement Homo sapiens
Mn2+ 25-80 mM: optimal concentration for free galactosylhydroxylysine as acceptor, 2 mM: optimal concentration for galactosylhydroxylysine bound in purified alpha1-chain of chicken skin collagen as acceptor, soluble enzyme is inhibited above 50 mM Homo sapiens
Mn2+ most effective metal cofactor Homo sapiens
additional information not activated by Ca2+ Homo sapiens
additional information not activated by Cd2+ Homo sapiens
additional information not activated by Zn2+, Cu2+ Homo sapiens
Ni2+ activates, soluble enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
80fold: soluble enzyme Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + [procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine = UDP + [procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine reaction mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet intact platelets and isolated plasma membranes Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0019
-
purified soluble enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-40°C, membrane-preparation, 1-2 months, stable Homo sapiens
-40°C, soluble enzyme after Biogel A-1.5M chromatography, 2-4 weeks, stable Homo sapiens
room temperature, soluble enzyme after DEAE-cellulose column, 18 h, 10% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: GDPglucose Homo sapiens ?
-
?
additional information not: ADPglucose Homo sapiens ?
-
?
TDP-glucose + D-galactosylhydroxylysine soluble enzyme, glucosylation at 60% of the rate of UDP-glucose Homo sapiens TDP + 1,2-D-glucosyl-D-galactosylhydroxylysine
-
?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen acetic acid-soluble bovine tendon collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen galactosylhydroxylysine bound in purified alpha1-chain of chicken skin collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen enzyme recognizes probably not only the carbohydrate side chains but also the primary structure of collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + D-galactosylhydroxylysine in vitro Homo sapiens UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine
-
?
UDP-glucose + D-galactosylhydroxylysine galactosylhydroxylysine purified from marine sponge collagen Homo sapiens UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
10% loss of activity within 18 h, soluble enzyme, after DEAE-ion exchange chromatography Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 soluble enzyme Homo sapiens
6.5 8
-
Homo sapiens
7 8 membrane-bound enzyme Homo sapiens