BRENDA - Enzyme Database show
show all sequences of 2.4.1.58

Biosynthesis of bacterial lipopolysaccharide. II. Incorporation of glucose and galactose catalyzed by particulate and soluble enzymes in Salmonella

Rothfield, L.; Osborn, M.J.; Horecker, B.L.; J. Biol. Chem. 239, 2788-2795 (1964)

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing: rapid loss of activity
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
lipopolysaccharide
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.074
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
most of the activity is located in the cell-wall fraction, but also significant activity in soluble fraction
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
soluble enzyme: MgCl2 stimulates more than MnCl2, optimal concentration: 0.02 M
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
soluble enzyme: MgCl2 stimulates more than MnCl2
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium SL797
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium SL797
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability
Storage Stability
Organism
-18°C or 4°C, soluble enzyme, overnight storage, 30-50% loss of activity
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
-
-
-
-
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to the backbone portion of lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to the backbone portion of lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
UDPglucose + lipopolysaccharide
transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
soluble enzyme, Tris buffer
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability (protein specific)
General Stability
Organism
freezing and thawing: rapid loss of activity
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
lipopolysaccharide
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.074
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
most of the activity is located in the cell-wall fraction, but also significant activity in soluble fraction
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
soluble enzyme: MgCl2 stimulates more than MnCl2, optimal concentration: 0.02 M
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
soluble enzyme: MgCl2 stimulates more than MnCl2
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium SL797
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability (protein specific)
Storage Stability
Organism
-18°C or 4°C, soluble enzyme, overnight storage, 30-50% loss of activity
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
-
-
-
-
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to the backbone portion of lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to the backbone portion of lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
UDPglucose + lipopolysaccharide
transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489298
Salmonella enterica subsp. enterica serovar Typhimurium SL797
UDP + D-glucosyllipopolysaccharide
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
soluble enzyme, Tris buffer
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 2.4.1.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719223
Twelkmeyer
The role of lic2B in lipopolys ...
Haemophilus influenzae, Haemophilus influenzae Eagan
Carbohydr. Res.
346
1262-1266
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720277
Perumal
Cloning and targeted disruptio ...
Pseudomonas aeruginosa
J. Mol. Microbiol. Biotechnol.
19
169-179
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703886
Schwingel
A unique glycosyltransferase i ...
Moraxella catarrhalis
Glycobiology
18
447-455
2008
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
489300
Creeger
Cloning of genes for bacterial ...
Escherichia coli, Escherichia coli K12 CS520, Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
254
804-810
1979
2
-
1
-
-
-
-
2
2
1
-
2
-
8
-
-
1
-
-
-
2
-
15
-
2
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
2
2
1
-
2
-
-
-
1
-
-
2
-
15
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
489212
Muller
Studies of phospholipid-requir ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium G30A
J. Biol. Chem.
247
2614-2622
1972
2
-
-
-
-
-
2
3
1
4
1
1
-
2
-
1
1
-
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
3
1
4
1
1
-
-
1
1
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489298
Rothfield
Biosynthesis of bacterial lipo ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SL797
J. Biol. Chem.
239
2788-2795
1964
-
-
-
-
-
1
-
2
1
2
-
2
-
2
-
-
-
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
2
-
2
-
-
-
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-