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Literature summary for 2.4.1.49 extracted from

  • Hiraishi, M.; Igarashi, K.; Kimura, S.; Wada, M.; Kitaoka, M.; Samejima, M.
    Synthesis of highly ordered cellulose II in vitro using cellodextrin phosphorylase (2009), Carbohydr. Res., 344, 2468-2473.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.81
-
cellotriose
-
Acetivibrio thermocellus
0.82
-
cellotetraose
-
Acetivibrio thermocellus
69
-
cellobiose
-
Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus YM4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + cellobiose
-
Acetivibrio thermocellus phosphate + cellotriose
-
?
alpha-D-glucose 1-phosphate + cellobiose
-
Acetivibrio thermocellus YM4 phosphate + cellotriose
-
?
alpha-D-glucose 1-phosphate + cellotetraose
-
Acetivibrio thermocellus phosphate + cellopentaose
-
?
alpha-D-glucose 1-phosphate + cellotetraose
-
Acetivibrio thermocellus YM4 phosphate + cellopentaose
-
?
alpha-D-glucose 1-phosphate + cellotriose
-
Acetivibrio thermocellus phosphate + cellotetraose
-
?
alpha-D-glucose 1-phosphate + cellotriose
-
Acetivibrio thermocellus YM4 phosphate + cellotetraose
-
?

Synonyms

Synonyms Comment Organism
cellodextrin phosphorylase
-
Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
cellobiose
-
Acetivibrio thermocellus
3.2
-
cellotetraose
-
Acetivibrio thermocellus
4
-
cellotriose
-
Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00174
-
cellobiose
-
Acetivibrio thermocellus
3.9
-
cellotetraose
-
Acetivibrio thermocellus
4.94
-
cellotriose
-
Acetivibrio thermocellus