Crystallization (Comment) | Organism |
---|---|
structures of wild-type and mutant D302C. Conserved active site residues Arg188 and Asp302 are critical for catalysis, and disruption of their hydrogen bond network through mutation can dramatically decrease enzymatic activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D302A | almost complete loss of activity | Homo sapiens |
D302A/D316A | very low residual activity | Homo sapiens |
D302C | kcat value is 9.5% that of wild-type GTB | Homo sapiens |
D302E | kcat value is 47% that of wild-type GTB | Homo sapiens |
D302E/D316E | almost complete loss of activity | Homo sapiens |
D302L | almost complete loss of activity | Homo sapiens |
D302N | almost complete loss of activity | Homo sapiens |
R188K | almost complete loss of activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
UDP-N-acetyl-alpha-D-galactosamine | wild-type, pH 7.0, 37°C | Homo sapiens | |
0.0099 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | wild-type, pH 7.0, 37°C | Homo sapiens | |
0.022 | - |
UDP-N-acetyl-alpha-D-galactosamine | mutant D302C, pH 7.0, 37°C | Homo sapiens | |
0.045 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302C, pH 7.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16422 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-galactosamine + alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | - |
Homo sapiens | UDP + alpha-D-GalNAc-(1->3)-[alpha-Fuc-(1->2)]-beta-D-Gal-(CH2)7CH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302C, pH 7.0, 37°C | Homo sapiens | |
17.5 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | wild-type, pH 7.0, 37°C | Homo sapiens |